ArticleAmerican journal of physiology. Cell physiology2015
Molecular determinants of force production in human skeletal muscle fibers: effects of myosin isoform expression and cross-sectional area.
Article in American journal of physiology. Cell physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01190046 (Muscle Disuse and Contractile Dysfunction in the Elderly), which is not on this map. Cited by 37 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Muscle Disuse and Contractile Dysfunction in the Elderly
Who cites it
37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 72 citations in OpenAlex.
- Effects of age on human skeletal muscle: a systematic review and meta-analysis of myosin heavy chain isoform protein expression, fiber size, and distribution.American journal of physiology. Cell physiology · 2024Pooled it
- Improvements in skeletal muscle fiber size with resistance training are age-dependent in older adults: a systematic review and meta-analysis.Journal of applied physiology (Bethesda, Md. : 1985) · 2020Pooled it
- The effects of resistance exercise training on arterial stiffness in metabolic syndrome.European journal of applied physiology · 2016Trial
- Isometric slow- and fast-contracting skeletal muscle fibres have more similar cellular and molecular properties at 37°C than at 25°C in older adults.The Journal of physiology · 2026Article
- Paraspinal muscle fibres demonstrate a complex relationship between contractile variability and function in canines with spinal degeneration.Scientific reports · 2025Article
- Pirfenidone treatment improves ischemic muscle function in mice with chronic kidney disease.Skeletal muscle · 2025Article
- Full Squats Enhance Performance and Body Composition, but Not Hypertrophy, Compared to Half Squats in Elite Young Tennis Players.Journal of functional morphology and kinesiology · 2025Article
- Sprinters' and Marathon Runners' Performances Are Better Explained by Muscle Fibers' Percentage Cross-Sectional Area than Any Other Parameter of Muscle Fiber Composition.Sports (Basel, Switzerland) · 2025Article
- Can non-invasive motor unit analysis reveal distinct neural strategies of force production in young with uncomplicated type 1 diabetes?European journal of applied physiology · 2025Article
- Fatiguing exercise reduces cellular passive Young's modulus in human vastus lateralis muscle.Experimental physiology · 2024Article
- The structural and functional effects of myosin regulatory light chain phosphorylation are amplified by increases in sarcomere length and [CaThe Journal of physiology · 2024Article
- Predicting myosin heavy chain isoform from postdissection fiber length in human skeletal muscle fibers.American journal of physiology. Cell physiology · 2024Article
- Baselining physiological parameters in three muscles across three equine breeds. What can we learn from the horse?Frontiers in physiology · 2024Article
- Muscle fiber phenotype: a culprit of abnormal metabolism and function in skeletal muscle of humans with obesity.American journal of physiology. Endocrinology and metabolism · 2023Review
- Automatic Text-Mining Approach to Identify Molecular Target Candidates Associated with Metabolic Processes for Myotonic Dystrophy Type 1.International journal of environmental research and public health · 2023Article
- Single muscle fibre contractile function with ageing.The Journal of physiology · 2022Review
- Artificial intelligence workflow quantifying muscle features on Hematoxylin-Eosin stained sections reveals dystrophic phenotype amelioration upon treatment.Scientific reports · 2022Article
- Iron Supplementation Improves Skeletal Muscle Contractile Properties in Mice with CKD.Kidney360 · 2022Article
- Review
- Inertial artifact in viscoelastic measurements of striated muscle: Modeling and experimental results.Biophysical journal · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Skeletal muscle contractile performance is governed by the properties of its constituent fibers, which are, in turn, determined by the molecular interactions of the myofilament proteins. To define the molecular determinants of contractile function in humans, we measured myofilament mechanics during maximal Ca(2+)-activated and passive isometric conditions in single muscle fibers with homogenous (I and IIA) and mixed (I/IIA and IIA/X) myosin heavy chain (MHC) isoforms from healthy, young adult male (n = 5) and female (n = 7) volunteers. Fibers containing only MHC II isoforms (IIA and IIA/X) produced higher maximal Ca(2+)-activated forces over the range of cross-sectional areas (CSAs) examined than MHC I fibers, resulting in higher (24-42%) specific forces. The number and/or stiffness of the strongly bound myosin-actin cross bridges increased in the higher force-producing MHC II isoforms and, in all isoforms, better predicted force than CSA. In men and women, cross-bridge kinetics, in terms of myosin attachment time and rate of myosin force production, were independent of CSA, although women had faster (7-15%) kinetics. The relative proportion of cross bridges and/or their stiffness was reduced as fiber size increased, causing a decline in specific force. Results from our examination of molecular mechanisms across the range of physiological CSAs explain the variation in specific force among the different fiber types in human skeletal muscle, which may have relevance to understanding how various physiological and pathophysiological conditions modulate single-fiber and whole muscle contractility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.