ArticleJournal of the Royal Society, Interface2015
Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates.
Article in Journal of the Royal Society, Interface, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Frequency-dependent cellular microrheology with pyramidal atomic force microscopy probes.Biophysical journal · 2026Article
- Microarchitected Compliant Scaffolds of Pyrolytic Carbon for 3D Muscle Cell Growth.Advanced healthcare materials · 2024Article
- Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment.Communications biology · 2023Review
- Epithelial cells sacrifice excess area to preserve fluidity in response to external mechanical stress.Communications biology · 2022Article
- Epithelial cells fluidize upon adhesion but display mechanical homeostasis in the adherent state.Biophysical journal · 2022Article
- Viscoelasticity of basal plasma membranes and cortices derived from MDCK II cells.Biophysical reports · 2021Article
- A designer cell culture insert with a nanofibrous membrane toward engineering an epithelial tissue model validated by cellular nanomechanics.Nanoscale advances · 2021Article
- Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing.International journal of molecular sciences · 2021Article
- Insights intoMaterials (Basel, Switzerland) · 2020Article
- The Research Advance of Cell BridgesFrontiers in bioengineering and biotechnology · 2020Review
- Stiffness of MDCK II Cells Depends on Confluency and Cell Size.Biophysical journal · 2019Article
- Formation of arrays of planar, murine, intestinal crypts possessing a stem/proliferative cell compartment and differentiated cell zone.Lab on a chip · 2018Article
- Viscoelastic Properties of Confluent MDCK II Cells Obtained from Force Cycle Experiments.Biophysical journal · 2017Article
- PeakForce Tapping resolves individual microvilli on living cells.Journal of molecular recognition : JMR · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The impact of substrate topography on the morphological and mechanical properties of confluent MDCK-II cells cultured on porous substrates was scrutinized by means of various imaging techniques as well as atomic force microscopy comprising force volume and microrheology measurements. Regardless of the pore size, ranging from 450 to 5500 nm in diameter, cells were able to span the pores. They did not crawl into the holes or grow around the pores. Generally, we found that cells cultured on non-porous surfaces are stiffer, i.e. cortical tension rises from 0.1 to 0.3 mN m(-1), and less fluid than cells grown over pores. The mechanical data are corroborated by electron microscopy imaging showing more cytoskeletal filaments on flat samples in comparison to porous ones. By contrast, cellular compliance increases with pore size and cells display a more fluid-like behaviour on larger pores. Interestingly, cells on pores larger than 3500 nm produce thick actin bundles that bridge the pores and thereby strengthen the contact zone of the cells.
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