ArticleBiophysical journal2015
Estimating orientation factors in the FRET theory of fluorescent proteins: the TagRFP-KFP pair and beyond.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Genetic Encoding of 3-Cyano-Tyrosine and Its Use in Controlling the Chromophore Isomeric State of the Fluorescent Protein mKate.International journal of molecular sciences · 2026Article
- Integrating Molecular Dynamics Simulations and Single-molecule FRET Spectroscopy: From Computational FRET Estimation to Experimental Data Interpretation.The journal of physical chemistry. B · 2026Review
- Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology.Journal of microscopy · 2025Review
- One-Step Homogeneous Immunoassay for the Detection of Influenza Virus Using Switching Peptide and Graphene Quencher.Biochip journal · 2022Article
- Directionality of light absorption and emission in representative fluorescent proteins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- DNA Origami as Emerging Technology for the Engineering of Fluorescent and Plasmonic-Based Biosensors.Materials (Basel, Switzerland) · 2020Review
- Novel combinations of experimental and computational analysis tested on the binding of metalloprotoporphyrins to albumin.International journal of biological macromolecules · 2019Article
- Two-Step Energy Transfer Dynamics in Conjugated Polymer and Dye-Labeled Aptamer-Based Potassium Ion Detection Assay.Polymers · 2019Article
- In-Vitro Characterization of mCerulean3_mRuby3 as a Novel FRET Pair with Favorable Bleed-Through Characteristics.Biosensors · 2019Article
- Long-Range Energy Transfer in Protein Megamolecules.Journal of the American Chemical Society · 2018Article
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
- Photodynamic Physiology-Photonanomanipulations in Cellular Physiology with Protein Photosensitizers.Frontiers in physiology · 2017Review
- Green fluorescent protein with anionic tryptophan-based chromophore and long fluorescence lifetime.Biophysical journal · 2015Article
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Authors and funding
4 authors.
Funding
Abstract
The orientation factor κ(2), one of the key parameters defining Förster resonance energy transfer efficiency, is determined by the transition dipole moment orientations of the donor and acceptor species. Using the results of quantum chemical and quantum mechanical/molecular mechanical calculations for the chromophore-containing pockets in selected colored proteins of the green fluorescent protein family, we derived transition dipole moments corresponding to the S0,min → S1 excitation for green fluorescent protein, red fluorescent protein (TagRFP), and kindling fluorescent protein, and the S1,min → S0 emission for TagRFP. These data allowed us to estimate κ(2) values for the TagRFP-linker-kindling fluorescent protein tetrameric complex required for constructing novel sensors.
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