ArticleBiophysical journal2015
Two-bubble acoustic tweezing cytometry for biomechanical probing and stimulation of cells.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Acoustic Bubble Sensing Techniques and Bioapplications.Biosensors · 2026Review
- Mechanoglycobiology: The Roles of Cell Surface Glycans in Mechanosensing and Mechanotransduction.Chembiochem : a European journal of chemical biology · 2025Article
- Metabolic-Glycoengineering-Enabled Molecularly Specific Acoustic Tweezing Cytometry for Targeted Mechanical Stimulation of Cell Surface Sialoglycans.Angewandte Chemie (International ed. in English) · 2024Article
- Rapid responses of human pluripotent stem cells to cyclic mechanical strains applied to integrin by acoustic tweezing cytometry.Scientific reports · 2023Article
- Low-intensity pulsed ultrasound/nanomechanical force generators enhance osteogenesis of BMSCs through microfilaments and TRPM7.Journal of nanobiotechnology · 2022Article
- Micro/nanoengineered technologies for human pluripotent stem cells maintenance and differentiation.Nano today · 2021Article
- Using Acoustic Fields to Fabricate ECM-Based Biomaterials for Regenerative Medicine Applications.Recent progress in materials · 2020Article
- Probing Single-Cell Mechanical Allostasis Using Ultrasound Tweezers.Cellular and molecular bioengineering · 2019Article
- A Toolbox for Organelle Mechanobiology Research-Current Needs and Challenges.Micromachines · 2019Article
- Acoustic tweezing cytometry for mechanical phenotyping of macrophages and mechanopharmaceutical cytotripsy.Scientific reports · 2019Article
- Production of acoustic radiation force using ultrasound: methods and applications.Expert review of medical devices · 2018Review
- Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation.Scientific reports · 2018Article
- Mechanogenetics for the remote and noninvasive control of cancer immunotherapy.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
- Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation.Biomaterials · 2017Article
- Single microtubules and small networks become significantly stiffer on short time-scales upon mechanical stimulation.Scientific reports · 2017Article
- Mixing high-viscosity fluids via acoustically driven bubbles.Journal of micromechanics and microengineering : structures, devices, and systems · 2017Article
- Biophysical Tools for Cellular and Subcellular Mechanical Actuation of Cell Signaling.Biophysical journal · 2016Review
- Ultrasound Therapy, Chemotherapy and Their Combination for Prostate Cancer.Technology in cancer research & treatmentReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The study of mechanotransduction relies on tools that are capable of applying mechanical forces to elicit and assess cellular responses. Here we report a new (to our knowledge) technique, called two-bubble acoustic tweezing cytometry (TB-ATC), for generating spatiotemporally controlled subcellular mechanical forces on live cells by acoustic actuation of paired microbubbles targeted to the cell adhesion receptor integrin. By measuring the ultrasound-induced activities of cell-bound microbubbles and the actin cytoskeleton contractile force responses, we determine that TB-ATC elicits mechanoresponsive cellular changes via cyclic, paired displacements of integrin-bound microbubbles driven by the attractive secondary acoustic radiation force (sARF) between the bubbles in an ultrasound field. We demonstrate the feasibility of dual-mode TB-ATC for both subcellular probing and mechanical stimulation. By exploiting the robust and unique interaction of ultrasound with microbubbles, TB-ATC provides distinct advantages for experimentation and quantification of applied forces and cellular responses for biomechanical probing and stimulation of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.