ArticleBiophysical journal2015
Action potential morphology of human induced pluripotent stem cell-derived cardiomyocytes does not predict cardiac chamber specificity and is dependent on cell density.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Article
- Large-scale synthetic data enable digital twins of human excitable cells.bioRxiv : the preprint server for biology · 2025Article
- Article
- Maturation of human induced pluripotent stem cell-derived cardiomyocytes promoted by Brachyury priming.Scientific reports · 2025Article
- Review
- Investigating Inherited Heart Diseases Using Human Induced Pluripotent Stem Cell-Based Models.Life (Basel, Switzerland) · 2024Review
- Stem cell-derived cardiomyocyte heterogeneity confounds electrophysiological insights.The Journal of physiology · 2024Review
- High-throughput methods for cardiac cellular electrophysiology studies: the road to personalized medicine.American journal of physiology. Heart and circulatory physiology · 2024Review
- Induced pluripotent stem cell-derived cardiomyocytes-more show than substance?Biophysical reviews · 2023Review
- Conductive electrospun polymer improves stem cell-derived cardiomyocyte function and maturation.Biomaterials · 2023Article
- Electrophysiological and calcium-handling development during long-term culture of human-induced pluripotent stem cell-derived cardiomyocytes.Basic research in cardiology · 2023Article
- Whole-cell patch-clamp recording and parameters.Biophysical reviews · 2023Article
- Human induced pluripotent stem cell-derived cardiomyocytes as an electrophysiological model: Opportunities and challenges-The Hamburg perspective.Frontiers in physiology · 2023Review
- Integrins Increase Sarcoplasmic Reticulum Activity for Excitation-Contraction Coupling in Human Stem Cell-Derived Cardiomyocytes.International journal of molecular sciences · 2022Article
- Nanocrown electrodes for parallel and robust intracellular recording of cardiomyocytes.Nature communications · 2022Article
- Article
- Isogenic Sets of hiPSC-CMs Harboring Distinct KCNH2 Mutations Differ Functionally and in Susceptibility to Drug-Induced Arrhythmias.Stem cell reports · 2020Article
- Syncytium cell growth increases Kir2.1 contribution in human iPSC-cardiomyocytes.American journal of physiology. Heart and circulatory physiology · 2020Article
- Optical mapping of human embryonic stem cell-derived cardiomyocyte graft electrical activity in injured hearts.Stem cell research & therapy · 2020Article
- Blinded, Multicenter Evaluation of Drug-induced Changes in Contractility Using Human-induced Pluripotent Stem Cell-derived Cardiomyocytes.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
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Abstract
Previous studies investigating human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have proposed the distinction of heart chamber-specific (atrial, ventricular, pacemaker) electrophysiological phenotypes based on action potential (AP) morphology. This suggestion has been based on data acquired using techniques that allow measurements from only a small number of cells and at low seeding densities. It has also been observed that density of culture affects the properties of iPSC-CMs. Here we systematically analyze AP morphology from iPSC-CMs at two seeding densities: 60,000 cells/well (confluent monolayer) and 15,000 cells/well (sparsely-seeded) using a noninvasive optical method. The confluent cells (n = 360) demonstrate a series of AP morphologies on a normally distributed spectrum with no evidence for specific subpopulations. The AP morphologies of sparsely seeded cells (n = 32) displayed a significantly different distribution, but even in this case there is no clear evidence of chamber-specificity. Reduction in gap junction conductance using carbenoxolone only minimally affected APD distribution in confluent cells. These data suggest that iPSC-CMs possess a sui generis AP morphology, and when observed in different seeding densities may encompass any shape including those resembling chamber-specific subtypes. These results may be explained by different functional maturation due to culture conditions.
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