ArticleThe Journal of cell biology1989
Transformation of murine melanocytes by basic fibroblast growth factor cDNA and oncogenes and selective suppression of the transformed phenotype in a reconstituted cutaneous environment.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 145 citations in OpenAlex.
- Transcriptional background effects on a tumor driver gene in different pigment cell types of medaka.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- Comparison of Xiphophorus and human melanoma transcriptomes reveals conserved pathway interactions.Pigment cell & melanoma research · 2018Article
- Sensitivity of Melanoma Cells to EGFR and FGFR Activation but Not Inhibition is Influenced by Oncogenic BRAF and NRAS Mutations.Pathology oncology research : POR · 2015Article
- Active Notch1 confers a transformed phenotype to primary human melanocytes.Cancer research · 2009Article
- Notch tumor suppressor function.Oncogene · 2008Review
- Metabotropic glutamate receptors (mGlus) and cellular transformation.Neuropharmacology · 2008Review
- Oncogenic activities of metabotropic glutamate receptor 1 (Grm1) in melanocyte transformation.Pigment cell & melanoma research · 2008Article
- Notch1 is a p53 target gene involved in human keratinocyte tumor suppression through negative regulation of ROCK1/2 and MRCKalpha kinases.Genes & development · 2007Article
- Mechanism of UV-related carcinogenesis and its contribution to nevi/melanoma.Expert review of dermatology · 2007Article
- Involvement of metabotropic glutamate receptor 1, a G protein coupled receptor, in melanoma development.Journal of molecular medicine (Berlin, Germany) · 2004Review
- Angiogenesis, lymphangiogenesis, and melanoma metastasis.Oncogene · 2003Review
- Uveal melanomas express vascular endothelial growth factor and basic fibroblast growth factor and support endothelial cell growth.The British journal of ophthalmology · 2002Article
- Absence of fibroblast growth factor 2 does not prevent tumor formation originating from the RPE.Oncogene · 2002Article
- Activation of STAT5 triggers proliferation and contributes to anti-apoptotic signalling mediated by the oncogenic Xmrk kinase.Oncogene · 2002Article
- Basic fibroblast growth factor and ultraviolet B transform melanocytes in human skin.The American journal of pathology · 2001Article
- Basic fibroblast growth factor induces a transformed phenotype in normal human melanocytes.Oncogene · 1999Article
- Suppression of autocrine and paracrine functions of basic fibroblast growth factor by stable expression of perlecan antisense cDNA.Molecular and cellular biology · 1997Article
- Growth requirements and neoplastic transformation of two types of normal human breast epithelial cells derived from reduction mammoplasty.In vitro cellular & developmental biology. Animal · 1997Article
- msg1, a novel melanocyte-specific gene, encodes a nuclear protein and is associated with pigmentation.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Transcriptional regulation of basic fibroblast growth factor gene by p53 in human glioblastoma and hepatocellular carcinoma cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Constitutive expression of basic fibroblast growth factor (bFGF), a common characteristic of metastatic melanomas, was reproduced in vitro by infection of normal murine melanocytes with a recombinant retrovirus carrying a cDNA for bFGF. Expression of bFGF in these cells conferred autonomous growth in culture and extinguished differentiated functions, such as the synthesis of melanin and formation of dendrites. Independence from exogenous bFGF and loss of differentiated functions in vitro were induced also by transformation of melanocytes with the oncogenes myc, Ela, ras, and neu, although bFGF was not expressed by the respective transformants. As shown in skin reconstitution experiments onto syngeneic mice and subcutaneous injections into nude mice, the various transformants differed in their behavior in vivo. The bFGF transformants did not form tumors. They reverted to having a normal, melanotic phenotype and restricted growth. Myc and Ela transformants grew as tumors in nude mice but not in syngeneic, immunocompetent animals. Ras-transformed melanocytes were always tumorigenic, whereas the formation of tumors by neu transformants was suppressed by the concomitant grafting of keratinocytes in reconstituted skin of syngeneic mice. These data show that melanocytes genetically manipulated to produce bFGF acquire properties in vitro similar to those of metastatic melanoma cells or those induced by various oncogenes but that constitutive production of bFGF by itself is insufficient to make melanocytes tumorigenic. The experiments also show that melanocytes transformed by the selected oncogenes respond differentially to various environments in vivo.
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