ArticleThe Journal of cell biology1989
cAMP-mediated inhibition of intracellular particle movement and actin reorganization in Dictyostelium.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 65 citations in OpenAlex.
- The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum.Cell motility and the cytoskeleton · 2009Article
- Intracellular role of adenylyl cyclase in regulation of lateral pseudopod formation during Dictyostelium chemotaxis.Eukaryotic cell · 2005Article
- RasC plays a role in transduction of temporal gradient information in the cyclic-AMP wave of Dictyostelium discoideum.Eukaryotic cell · 2004Article
- Constitutively active protein kinase A disrupts motility and chemotaxis in Dictyostelium discoideum.Eukaryotic cell · 2003Article
- The internal phosphodiesterase RegA is essential for the suppression of lateral pseudopods during Dictyostelium chemotaxis.Molecular biology of the cell · 2000Article
- Capping protein terminates but does not initiate chemoattractant-induced actin assembly in Dictyostelium.The Journal of cell biology · 1997Article
- Chemotaxis to cAMP and slug migration in Dictyostelium both depend on migA, a BTB protein.Molecular biology of the cell · 1997Article
- Actin-based organelle movement.Experientia · 1996Review
- Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants.Molecular biology of the cell · 1996Article
- Ponticulin plays a role in the positional stabilization of pseudopods.The Journal of cell biology · 1995Article
- Organelle-cytoskeletal interactions: actin mutations inhibit meiosis-dependent mitochondrial rearrangement in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- Actin-dependent mitochondrial motility in mitotic yeast and cell-free systems: identification of a motor activity on the mitochondrial surface.The Journal of cell biology · 1995Article
- A density-sensing factor regulates signal transduction in Dictyostelium.The Journal of cell biology · 1995Article
- Cell-substrate interactions and locomotion of Dictyostelium wild-type and mutants defective in three cytoskeletal proteins: a study using quantitative reflection interference contrast microscopy.Biophysical journal · 1995Article
- Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity.Molecular biology of the cell · 1994Article
- Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.Molecular biology of the cell · 1993Article
- The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility.Molecular biology of the cell · 1993Article
- Targeted disruption of the ABP-120 gene leads to cells with altered motility.The Journal of cell biology · 1992Article
- Myosin II heavy chain null mutant of Dictyostelium exhibits defective intracellular particle movement.The Journal of cell biology · 1990Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Before addition of cAMP, Dictyostelum amoebae rapidly translocating in buffer are elongate, exhibit expansion zones primarily at the anterior end and filamentous actin (F-actin) localization primarily in the anterior pseudopodia. Intracellular particle movement is primarily in the anterior direction, and the average rate of particle movement is roughly five times the rate of cellular translocation. Within seconds after the addition of 10(-6)M cAMP, there is a dramatic suppression of cellular translocation, an inhibition of pseudopod formation, a freeze in cellular morphology, a dramatic depression in intracellular particle movement, loss of F-actin localization in pseudopodia concomitant with relocalization of F-actin in the general cytoplasmic cortex under the plasma membrane, and a doubling of F-actin content. After 10 s, expansion zones are again visible at the cell perimeter, but they no longer are localized in the original anterior portion of the cell. There is a slight rebound in particle movement after 10 s, but particles with persistent tracks now show no directionality towards the original anterior portion of the cell, as they did before cAMP addition. Finally, in parallel with the resumption of peripheral expansion and the small rebound in particle movement, there is a decrease in total cellular F-actin to the untreated level. The pattern of microtubule organization is unaffected by the addition of cAMP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.