ArticleInternational journal of biological sciences2015
Enhancement of larval RNAi efficiency by over-expressing Argonaute2 in Bombyx mori.
Article in International journal of biological sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 51 citations in OpenAlex.
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae.Genetics · 2026Article
- The Co-Delivery of Natural Products and Small RNAs for Cancer Therapy: A Review.Molecules (Basel, Switzerland) · 2025Review
- RNAi turns 25:contributions and challenges in insect science.Frontiers in insect science · 2023Review
- Dichotomous sperm in Lepidopteran insects: a biorational target for pest management.Frontiers in insect science · 2023Review
- Increased RNAi Efficiency by dsInsects · 2022Article
- Long Non-Coding RNAInsects · 2022Article
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
- RNAi-based pest control: Production, application and the fate of dsRNA.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Next Generation dsRNA-Based Insect Control: Success So Far and Challenges.Frontiers in plant science · 2021Review
- Double-Stranded RNA Binding Proteins in Serum Contribute to Systemic RNAi Across Phyla-Towards Finding the Missing Link in Achelata.International journal of molecular sciences · 2020Article
- Mechanisms, Applications, and Challenges of Insect RNA Interference.Annual review of entomology · 2020Review
- A Novel Splice Variant of the Masculinizing GeneBiomolecules · 2019Article
- Three Melanin Pathway Genes,International journal of molecular sciences · 2019Article
- Molecular Characterization and the Function ofInternational journal of molecular sciences · 2018Article
- Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines.Scientific reports · 2018Article
- RNA Interference in Insects: Protecting Beneficials and Controlling Pests.Frontiers in physiology · 2018Review
- Comparative analysis of double-stranded RNA degradation and processing in insects.Scientific reports · 2017Article
- Implementing the sterile insect technique with RNA interference - a review.Entomologia experimentalis et applicata · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference has been described as a powerful genetic tool for gene functional analysis and a promising approach for pest management. However, RNAi efficiency varies significantly among insect species due to distinct RNAi machineries. Lepidopteran insects include a large number of pests as well as model insects, such as the silkworm, Bombyx mori. However, only limited success of in vivo RNAi has been reported in lepidoptera, particularly during the larval stages when the worms feed the most and do the most harm to the host plant. Enhancing the efficiency of larval RNAi in lepidoptera is urgently needed to develop RNAi-based pest management strategies. In the present study, we investigate the function of the conserved RNAi core factor, Argonaute2 (Ago2), in mediating B. mori RNAi efficiency. We demonstrate that introducing BmAgo2 dsRNA inhibits the RNAi response in both BmN cells and embryos. Furthermore, we establish several transgenic silkworm lines to assess the roles of BmAgo2 in larval RNAi. Over-expressing BmAgo2 significantly facilitated both dsRNA-mediated larval RNAi when targeting DsRed using dsRNA injection and shRNA-mediated larval RNAi when targeting BmBlos2 using transgenic shRNA expression. Our results show that BmAgo2 is involved in RNAi in B. mori and provides a promising approach for improving larval RNAi efficiency in B. mori and in lepidopteran insects in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.