ArticleChinese journal of cancer research = Chung-kuo yen cheng yen chiu2014
Knockdown of HMGB1 improves apoptosis and suppresses proliferation and invasion of glioma cells.
Article in Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Ferroptosis inhibits biological behaviors of glioma cells by downregulating Galectin-9 transcriptional level via extracellular Acetyl-HMGB1.Scientific reports · 2026Article
- Article
- miR-665 is downregulated in glioma and inhibits tumor cell proliferation, migration and invasion by targeting high mobility group box 1.Oncology letters · 2021Article
- Functional Diversity of Non-Histone Chromosomal Protein HmgB1.International journal of molecular sciences · 2020Review
- MicroRNA-505-3p inhibits development of glioma by targeting HMGB1 and regulating AKT expression.Oncology letters · 2020Article
- The association between high mobility group box 1 chromatin protein and mitotic chromosomes in glioma cells.Oncology letters · 2020Article
- Emerging Pathogenic and Prognostic Significance of Paired Box 3 (PAX3) Protein in Adult Gliomas.Translational oncology · 2019Article
- Upregulated necroptosis-pathway-associated genes are unfavorable prognostic markers in low-grade glioma and glioblastoma multiforme.Translational cancer research · 2019Article
- MicroRNA-10b-5p downregulation inhibits the invasion of glioma cells via modulating homeobox B3 expression.Experimental and therapeutic medicine · 2019Article
- MicroRNA-142-3p inhibits proliferation and induces apoptosis by targeting the high-mobility group box 1 via the Wnt/β-catenin signaling pathway in glioma.International journal of clinical and experimental pathology · 2018Article
- Paradoxical Role of High Mobility Group Box 1 in Glioma: A Suppressor or a Promoter?Oncology reviews · 2017Review
- Pivotal role of high-mobility group box 1 (HMGB1) signaling pathways in glioma development and progression.Journal of molecular medicine (Berlin, Germany) · 2016Review
- MicroRNA-218 modulates activities of glioma cells by targeting HMGB1.American journal of translational research · 2016Article
- Role of high-mobility group box 1 in methamphetamine-induced activation and migration of astrocytes.Journal of neuroinflammation · 2015Article
- MiR-129-2 functions as a tumor suppressor in glioma cells by targeting HMGB1 and is down-regulated by DNA methylation.Molecular and cellular biochemistry · 2015Article
- Hypoxia-induced HMGB1 in would tissues promotes the osteoblast cell proliferation via activating ERK/JNK signaling.International journal of clinical and experimental medicine · 2015Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purposes of this study were to explore the effects of high mobility group protein box 1 (HMGB1) gene on the growth, proliferation, apoptosis, invasion, and metastasis of glioma cells, with an attempt to provide potential therapeutic targets for the treatment of glioma.
methodsThe expressions of HMGB1 in glioma cells (U251, U-87MG and LN-18) and one control cell line (SVG p12) were detected by real time PCR and Western blotting, respectively. Then, the effects of HMGB1 on the biological behaviors of glioma cells were detected: the expression of HMGB1 in human glioma cell lines U251 and U-87MG were suppressed using RNAi technique, then the influences of HMGB1 on the viability, cycle, apoptosis, and invasion abilities of U251 and U-87MG cells were analyzed using in a Transwell invasion chamber. Also, the effects of HMGB1 on the expressions of cyclin D1, Bax, Bcl-2, and MMP 9 were detected.
resultsAs shown by real-time PCR and Western blotting, the expression of HMGB1 significantly increased in glioma cells (U251, U-87MG, and LN-18) in comparison with the control cell line (SVG p12); the vitality, proliferation and invasive capabilities of U251 and U-87MG cells in the HMGB1 siRNA-transfected group were significantly lower than those in the blank control group and negative control (NC) siRNA group (P<0.05) but showed no significant difference between the blank control group and NC siRNA group. The percentage of apoptotic U251 and U-87MG cells was significantly higher in the HMGB1 siRNA-transfected group than in the blank control group and NC siRNA group (P<0.05) but was similar between the latter two groups. The HMGB1 siRNA-transfected group had significantly lower expression levels of Cyclin D1, Bcl-2, and MMP-9 protein in U251 and U-87MG cells and significantly higher expression of Bax protein than in the blank control group and NC siRNA group (P<0.05); the expression profiles of cyclin D1, Bax, Bcl-2, and MMP 9 showed no significant change in both blank control group and NC siRNA group.
conclusionsHMGB1 gene may promote the proliferation and migration of glioma cells and suppress its effects of apoptosis. Inhibition of the expression of HMGB1 gene can suppress the proliferation and migration of glioma cells and promote their apoptosis. Our observations provided a new target for intervention and treatment of glioma.
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