ArticleProceedings of the National Academy of Sciences of the United States of America2015
Single-cell, real-time detection of oxidative stress induced in Escherichia coli by the antimicrobial peptide CM15.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed.
- OxyR regulates the oxidative stress response inApplied and environmental microbiology · 2026Article
- Membrane-Traversing Peptides: Mechanistic Landscapes, Assays, and Applications.Chemical reviews · 2026Review
- Bat-derived antimicrobial peptide MC-CATH3 Confers protection against bacterial infections via a potential ferroptosis-like death process.The Journal of biological chemistry · 2026Article
- Antimicrobial peptoids pass rapidly through bacterial membranes and flocculate ribosomes and DNA: A single-cell fluorescence study.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Overcoming methicillin resistance in Staphylococcus aureus via metal ion disruption of bacterial metabolism.npj antimicrobials and resistance · 2026Article
- Synergistic action of peptidoglycan and teichoic acid synthesis inhibitors leads to cell death by oxidative damage.Communications biology · 2026Article
- Overcoming multidrug resistance Acinetobacter baumanii via downregulated virulence factor genes by green fabricated silver Nanoparticles mediated Piper nigrum.Scientific reports · 2026Article
- Fluorescein-based dyes are not valid reporters of oxidative stress in bacteria, and conclusions based on their use must be reconsidered.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- High Concentrations of Non-Esterified Fatty Acids During BovineJournal of developmental biology · 2025Article
- Data-Independent Acquisition (DIA)-Based Label-Free Redox Proteomics (DIALRP) Identifies Prominent Cysteine Oxidations in Translation Machinery in Prostate Cancer Cells Under Oxidative Stress.Journal of proteome research · 2025Article
- Antibiotic fosmidomycin protects bacteria from cell wall perturbations by antagonizing oxidative damage-mediated cell lysis.Frontiers in microbiology · 2025Article
- Proton Tunneling Allows a Proton-Coupled Electron Transfer Process in the Cancer Cell.JACS Au · 2024Article
- A novel cathelicidin TS-CATH derived fromComputational and structural biotechnology journal · 2024Article
- Class IIb Microcin MccM Interferes with Oxidative Phosphorylation inACS chemical biology · 2024Article
- Synergistic collaboration between AMPs and non-direct antimicrobial cationic peptides.Nature communications · 2024Article
- Hydantoin derivative dimers as broad-spectrum antimicrobial agents against ESKAPE pathogens with enhanced killing rate and stability.RSC medicinal chemistry · 2024Article
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- PreventingACS applied materials & interfaces · 2024Article
- Fighting Microbial Infections fromAntibiotics (Basel, Switzerland) · 2024Article
19 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
Abstract
Antibiotics target specific biochemical mechanisms in bacteria. In response to new drugs, pathogenic bacteria rapidly develop resistance. In contrast, antimicrobial peptides (AMPs) have retained broad spectrum antibacterial potency over millions of years. We present single-cell fluorescence assays that detect reactive oxygen species (ROS) in the Escherichia coli cytoplasm in real time. Within 30 s of permeabilization of the cytoplasmic membrane by the cationic AMP CM15 [combining residues 1-7 of cecropin A (from moth) with residues 2-9 of melittin (bee venom)], three fluorescence signals report oxidative stress in the cytoplasm, apparently involving O2 (-), H2O2, and •OH. Mechanistic studies indicate that active respiration is a prerequisite to the CM15-induced oxidative damage. In anaerobic conditions, signals from ROS are greatly diminished and the minimum inhibitory concentration increases 20-fold. Evidently the natural human AMP LL-37 also induces a burst of ROS. Oxidative stress may prove a significant bacteriostatic mechanism for a variety of cationic AMPs. If so, host organisms may use the local oxygen level to modulate AMP potency.
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