ArticleInternational journal of molecular sciences2014
Exploring the Arabidopsis proteome: influence of protein solubilization buffers on proteome coverage.
Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Citrullination of Proteins as a Specific Response Mechanism in Plants.Frontiers in plant science · 2021Article
- Changes in the Arabidopsis RNA-binding proteome reveal novel stress response mechanisms.BMC plant biology · 2019Article
- Drought Stress Causes Specific Changes to the Spliceosome and Stress Granule Components.Frontiers in molecular biosciences · 2019Article
- Ethylene Receptors, CTRs and EIN2 Target Protein Identification and Quantification Through Parallel Reaction Monitoring During Tomato Fruit Ripening.Frontiers in plant science · 2018Article
- Article
- Assessing the Risk of Probiotic Dietary Supplements in the Context of Antibiotic Resistance.Frontiers in microbiology · 2017Article
- The RNA-binding protein repertoire of Arabidopsis thaliana.Scientific reports · 2016Article
- Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism.International journal of molecular sciences · 2016Article
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Authors and funding
8 authors.
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Abstract
The study of proteomes provides new insights into stimulus-specific responses of protein synthesis and turnover, and the role of post-translational modifications at the systems level. Due to the diverse chemical nature of proteins and shortcomings in the analytical techniques used in their study, only a partial display of the proteome is achieved in any study, and this holds particularly true for plant proteomes. Here we show that different solubilization and separation methods have profound effects on the resulting proteome. In particular, we observed that the type of detergents employed in the solubilization buffer preferentially enriches proteins in different functional categories. These include proteins with a role in signaling, transport, response to temperature stimuli and metabolism. This data may offer a functional bias on comparative analysis studies. In order to obtain a broader coverage, we propose a two-step solubilization protocol with first a detergent-free buffer and then a second step utilizing a combination of two detergents to solubilize proteins.
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