ArticleMolecular reproduction and development2015
RNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation.
Article in Molecular reproduction and development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis.International journal of molecular sciences · 2023Pooled it
- EFNA5 as an Oocyte-Derived Factor Enhances Developmental Competence by Modulating Oxidative Stress, Inflammation, and Apoptosis During In Vitro Maturation.Antioxidants (Basel, Switzerland) · 2025Article
- Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro.Reproduction (Cambridge, England) · 2025Article
- The dominant follicle: the final frontier in bovine oocyte development.Animal reproduction · 2025Article
- Identification of novel cattle (Bos taurus) genes and biological insights of their function in pre-implantation embryo development.BMC genomics · 2024Article
- Meiotic maturation failure in primary ovarian insufficiency: insights from a bovine model.Journal of assisted reproduction and genetics · 2024Article
- Single-cell profiling reveals transcriptome dynamics during bovine oocyte growth.BMC genomics · 2024Article
- Article
- Cathepsin L regulates oocyte meiosis and preimplantation embryo development.Cell proliferation · 2024Article
- RNA sequencing and gene co-expression network ofAnimal reproduction · 2024Article
- Expression analysis suggests that DNMT3L is required for oocyte de novo DNA methylation only in Muridae and Cricetidae rodents.Epigenetics & chromatin · 2023Article
- Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after In Vitro Maturation.International journal of molecular sciences · 2023Article
- RNA-Seq Analysis Identifies Differentially Expressed Genes in theInternational journal of molecular sciences · 2022Article
- Genome-wide DNA methylation differences between conservation and breeding populations of Shaoxing ducks.Heliyon · 2022Article
- Supplementation of SDF1 during Pig Oocyte In Vitro Maturation Improves Subsequent Embryo Development.Molecules (Basel, Switzerland) · 2022Article
- The impact of maternal age on gene expression during the GV to MII transition in euploid human oocytes.Human reproduction (Oxford, England) · 2021Article
- Isolation of high-quality total RNA and RNA sequencing of single bovine oocytes.STAR protocols · 2021Article
- A Transcriptomic Analysis of Gonads from the Low-Temperature-Induced Masculinization ofAnimals : an open access journal from MDPI · 2021Article
- Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes.Genes · 2021Article
- Essential shared and species-specific features of mammalian oocyte maturation-associated transcriptome changes impacting oocyte physiology.American journal of physiology. Cell physiology · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Molecular changes occurring during mammalian oocyte maturation are partly regulated by cytoplasmic polyadenylation (CP) and affect oocyte quality, yet the extent of CP activity during oocyte maturation remains unknown. Single bovine oocyte RNA sequencing (RNA-Seq) was performed to examine changes in transcript abundance during in vitro oocyte maturation in cattle. Polyadenylated RNA from individual germinal-vesicle and metaphase-II oocytes was amplified and processed for Illumina sequencing, producing approximately 30 million reads per replicate for each sample type. A total of 10,494 genes were found to be expressed, of which 2,455 were differentially expressed (adjusted P < 0.05 and fold change >2) between stages, with 503 and 1,952 genes respectively increasing and decreasing in abundance. Differentially expressed genes with complete 3'-untranslated-region sequence (279 increasing and 918 decreasing in polyadenylated transcript abundance) were examined for the presence, position, and distribution of motifs mediating CP, revealing enrichment (85%) and lack thereof (18%) in up- and down-regulated genes, respectively. Examination of total and polyadenylated RNA abundance by quantitative PCR validated these RNA-Seq findings. The observed increases in polyadenylated transcript abundance within the RNA-Seq data are likely due to CP, providing novel insight into targeted transcripts and resultant differential gene expression profiles that contribute to oocyte maturation.
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