ArticleNature chemical biology2015
Opposing effects of folding and assembly chaperones on evolvability of Rubisco.
Article in Nature chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Machine learning-assisted directed evolution of plant Rubisco.Science advances · 2026Article
- The fitness landscape of a form II rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance.Science advances · 2026Article
- High-Throughput Detection of Cyanobacterial Form I Rubisco Assembly.ACS synthetic biology · 2026Article
- Directed evolution of a plant Rubisco chaperone with altered client recognition.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Bi-handed assembly chaperones regulate protein complex assembly through an intramolecular handover mechanism.Science advances · 2025Article
- Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity.Nature plants · 2025Article
- Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.Plant biotechnology journal · 2025Article
- A Cyanobacterial Screening Platform for Rubisco Mutant Variants.ACS synthetic biology · 2025Article
- In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Discussion of the possibility of increasing the efficiency of photosynthesis using genetic and metabolic engineering methods.Biodesign research · 2025Review
- Autotrophic bacterial production of polyhydroxyalkanoates using carbon dioxide as a sustainable carbon source.Frontiers in bioengineering and biotechnology · 2025Review
- Rubisco is evolving for improved catalytic efficiency and COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024Review
- The small subunit of Rubisco and its potential as an engineering target.Journal of experimental botany · 2023Review
- Phosphoribulokinase abundance is not limiting the Calvin-Benson-Bassham cycle inFrontiers in plant science · 2023Article
- Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria.Biotechnology for biofuels · 2021Review
- Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for COBioresources and bioprocessing · 2021Article
- Rubisco Adaptation Is More Limited by Phylogenetic Constraint Than by Catalytic Trade-off.Molecular biology and evolution · 2021Article
- Exploring the oxygenase function of Form II Rubisco for production of glycolate from COAMB Express · 2021Article
- The Coevolution of RuBisCO, Photorespiration, and Carbon Concentrating Mechanisms in Higher Plants.Frontiers in plant science · 2021Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) catalyzes the fixation of CO2 in photosynthesis. Despite its pivotal role, Rubisco is an inefficient enzyme and thus is a key target for directed evolution. Rubisco biogenesis depends on auxiliary factors, including the GroEL/ES-type chaperonin for folding and the chaperone RbcX for assembly. Here we performed directed evolution of cyanobacterial form I Rubisco using a Rubisco-dependent Escherichia coli strain. Overexpression of GroEL/ES enhanced Rubisco solubility and tended to expand the range of permissible mutations. In contrast, the specific assembly chaperone RbcX had a negative effect on evolvability by preventing a subset of mutants from forming holoenzyme. Mutation F140I in the large Rubisco subunit, isolated in the absence of RbcX, increased carboxylation efficiency approximately threefold without reducing CO2 specificity. The F140I mutant resulted in a ∼55% improved photosynthesis rate in Synechocystis PCC6803. The requirement of specific biogenesis factors downstream of chaperonin may have retarded the natural evolution of Rubisco.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.