ArticlePlant cell reports2015
Effect of Agrobacterium strain and plasmid copy number on transformation frequency, event quality and usable event quality in an elite maize cultivar.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Induction of Synthetic Apomixis in Two Sorghum Hybrids Enables Seed Yield and Genotype Preservation Over Multiple Generations.Plant biotechnology journal · 2026Article
- Binary vector origin predictably determinesbioRxiv : the preprint server for biology · 2025Article
- Binary vector copy number engineering improves Agrobacterium-mediated transformation.Nature biotechnology · 2025Article
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Risk assessment and bioburden evaluation of Agrobacterium tumefaciens-mediated transient protein expression in plants using the CaMV35S promoter.BMC biotechnology · 2023Article
- An extensible vector toolkit and parts library for advanced engineering of plant genomes.The plant genome · 2023Article
- An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation.Transgenic research · 2022Article
- Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum.Communications biology · 2022Article
- Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators.Frontiers in plant science · 2022Article
- Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology.Plant physiology · 2021Review
- Developing a rapid and highly efficient cowpea regeneration, transformation and genome editing system using embryonic axis explants.The Plant journal : for cell and molecular biology · 2021Article
- High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns.Plant communications · 2021Article
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- Maize BIG GRAIN1 homolog overexpression increases maize grain yield.Plant biotechnology journal · 2020Article
- Characterization of DvSSJ1 transcripts targeting the smooth septate junction (SSJ) of western corn rootworm (Diabrotica virgifera virgifera).Scientific reports · 2020Article
- Review
- High efficiency Agrobacterium-mediated site-specific gene integration in maize utilizing the FLP-FRT recombination system.Plant biotechnology journal · 2019Article
- Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.Plant biotechnology journal · 2019Review
- Co-culturing on dry filter paper significantly increased the efficiency ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.Transgenic research · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageImproving Agrobacterium -mediated transformation frequency and event quality by increasing binary plasmid copy number and appropriate strain selection is reported in an elite maize cultivar. Agrobacterium-mediated maize transformation is a well-established method for gene testing and for introducing useful traits in a commercial biotech product pipeline. To develop a highly efficient maize transformation system, we investigated the effect of two Agrobacterium tumefaciens strains and three different binary plasmid origins of replication (ORI) on transformation frequency, vector backbone insertion, single copy event frequency (percentage of events which are single copy for all transgenes), quality event frequency (percentage of single copy events with no vector backbone insertions among all events generated; QE) and usable event quality frequency (transformation frequency times QE frequency; UE) in an elite maize cultivar PHR03. Agrobacterium strain AGL0 gave a higher transformation frequency, but a reduced QE frequency than LBA4404 due to a higher number of vector backbone insertions. Higher binary plasmid copy number positively correlated with transformation frequency and usable event recovery. The above findings can be exploited to develop high-throughput transformation protocols, improve the quality of transgenic events in maize and other plants.
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Registered trials
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