Evidence map›Paper›PMID 25558394›Full record

ArticleActa naturae2014

Specific visualization of tumor cells using upconversion nanophosphors.

E A Grebenik, A N Generalova, A V Nechaev, E V Khaydukov, K E Mironova, O A Stremovskiy, E N Lebedenko, A V Zvyagin, S M Deyev

Open access · bronzeAbstract read
PubMed Full text at PMC
In one paragraph

Article in Acta naturae, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Radioactive (Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

E A GrebenikShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
A N GeneralovaShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
A V NechaevLomonosov Moscow State University, GSP-1, Leninskie Gory, Moscow, 119991, Russia.
E V KhaydukovInstitute of Laser and Information Technologies, Russian Academy of Sciences, Pionerskaya Str., 2, Troitsk, 142190, Russia.
K E MironovaShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
O A StremovskiyShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
E N LebedenkoShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
A V ZvyaginShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia ; Lobachevsky Nizhniy Novgorod State University, Gagarina Prospekt, 23, Nizhniy Novgorod, 603950, Russia ; Department of Physics and Astronomy, Macquarie University, Sydney, NSW 2109, Australia.
S M DeyevShemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia ; Lobachevsky Nizhniy Novgorod State University, Gagarina Prospekt, 23, Nizhniy Novgorod, 603950, Russia.
Institute of Bioorganic Chemistry · RUInstitute on Laser and Information Technologies · RUMoscow State University · TJ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the "transparency window" of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase : barstar (Bn : Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn : UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.

Indexed as

anti-tumor antibodiesbiomarker imagingHER2self-assemblyupconversion nanophosphors

Identifiers

PMID25558394
PMCPMC4273091
OpenAlexW2957352534

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.