ArticleBiostatistics, bioinformatics and biomathematics2013
An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis.
Article in Biostatistics, bioinformatics and biomathematics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07722637 (Evaluation of Ferroptosis-Related Biomarkers in Individuals With Coronary Artery Disease), which is not on this map. Cited by 1,179 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Evaluation of Ferroptosis-Related Biomarkers in Individuals With Coronary Artery Disease: The Impact of Cardiac Rehabilitation
Who cites it
1,179 citing papers in PubMed.
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1,119 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
backgroundThe 2
resultsOur method takes into account the PCR efficiency of each individual sample. In addition, it eliminates the need for background fluorescence estimation or subtraction because the background can be cancelled out using the differencing strategy. The DNA amount for a certain gene and the relative DNA amount among different samples estimated using our method were closer to the true values compared to the results of the 2
conclusionsThe improved method, the individual efficiency corrected calculation, produces more accurate estimates in relative gene expression than the 2
Identifiers
25558171PMC4280562What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.