Evidence map›Paper›PMID 25557984›Full record

ArticleAndrologia2015

Recombinant PAI-1 therapy restores myoendothelial junctions and erectile function in PAI-1-deficient mice.

P K Kavoussi, K Heberlein, A C Straub, G J Lowe, J L Oliver, R P Smith, W D Steers, B H Annex, B E Isakson, J J Lysiak

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Andrologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026
    Review
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

P K KavoussiDepartment of Urology, University of Virginia Health System, Charlottesville, VA, USA.
K HeberleinDepartment of Molecular Physiology and Biological Physics, University of Virginia Health System, Charlottesville, VA, USA.
A C StraubRobert M. Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, VA, USA.
G J LoweDepartment of Urology, University of Virginia Health System, Charlottesville, VA, USA.
J L OliverDepartment of Urology, University of Virginia Health System, Charlottesville, VA, USA.
R P SmithDepartment of Urology, University of Virginia Health System, Charlottesville, VA, USA.
W D SteersDepartment of Urology, University of Virginia Health System, Charlottesville, VA, USA.
B H AnnexRobert M. Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, VA, USA.
B E IsaksonDepartment of Molecular Physiology and Biological Physics, University of Virginia Health System, Charlottesville, VA, USA.
J J LysiakDepartment of Urology, University of Virginia Health System, Charlottesville, VA, USA.
University of Virginia Health System · US

Funding

Mechanisms of Heterocellular Signaling at the Myoenothelial JunctionR01HL088554 · NHLBI · UNIVERSITY OF VIRGINIA · PI ISAKSON, BRANT E, WEISS, MITCHELL J · 2008 to 2017
$3.8M
Cell Clearance in Urogenital TractR01HD074981 · NICHD · UNIVERSITY OF VIRGINIA · PI LYSIAK, JEFFREY J, RAVICHANDRAN, KODI S · 2013 to 2017
$1.6M
NHLBI NIH HHS HL088554NHLBI NIH HHS R01 HL088554NICHD NIH HHS R01 HD074981
6 · The paper itself

Abstract

Myoendothelial junctions are specialised projections of cell : cell contact through the internal elastic lamina between endothelial cells and vascular smooth muscle cells. These junctions allow for endothelial cells and vascular smooth muscle cells to make direct membrane apposition and are involved in cell : cell communication. In this study, we evaluated for the presence of myoendothelial junctions in murine corporal tissue and used plasminogen activator inhibitor (PAI)-1-deficient mice, which lack myoendothelial junctions, to determine whether myoendothelial junctions affect erectile function. Transmission electron microscopy demonstrated the presence of myoendothelial junctions in the corporal tissue of wild-type mice and confirmed the decreased junction numbers in the tissue of PAI-1(-/-) mice. A potential role for myoendothelial junctions in tumescence was established; in that, PAI-1(-/-) mice demonstrated a significantly longer time to achieve maximal intracavernous pressure. Treatment of PAI-1(-/-) mice with recombinant PAI-1 restored the number of myoendothelial junctions in the corporal tissue and also induced a significant decrease in time to maximal corporal pressures. Myoendothelial junctions were similarly identified in the human corporal tissue. These results suggest a critical role for myoendothelial junctions in erectile pathophysiology and therapies aimed at restoring myoendothelial junction numbers in the corporal tissue may provide a novel therapy for erectile dysfunction.

Indexed as

AnimalsCell CommunicationEndothelium, VascularErectile DysfunctionIntercellular JunctionsMaleMiceMice, Inbred C57BLMice, KnockoutMicroscopy, Electron, TransmissionMuscle, Smooth, VascularPenile ErectionRecombinant ProteinsSerpin E2Recombinant ProteinsSerpin E2Serpine2 protein, mouseCell communicationendothelial cellserectile dysfunctionplasminogen activatorsvascular smooth muscle

Identifiers

PMID25557984
PMCPMC4485985
OpenAlexW1966236354

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.