Evidence map›Paper›PMID 25557550›Full record

ArticleMolecular cell2015

Dicer-TRBP complex formation ensures accurate mammalian microRNA biogenesis.

Ross C Wilson, Akshay Tambe, Mary Anne Kidwell, Cameron L Noland, Catherine P Schneider, Jennifer A Doudna

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers.

0numbers the graph read from it
0cells of the map it votes in
157citing papers in PubMed
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

157 citing papers in PubMed, 274 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. MicroRNAs' Impact on Heart Diseases.International journal of molecular sciences · 2025
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Review
  16. The biogenesis and regulation of animal microRNAs.Nature reviews. Molecular cell biology · 2025
    Review
  17. Review
  18. Review
  19. Competition effects regulating the composition of the microRNA pool.Journal of the Royal Society, Interface · 2025
    Article
  20. Article

97 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ross C WilsonDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Akshay TambeDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Mary Anne KidwellDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Cameron L NolandDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Catherine P SchneiderDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Jennifer A DoudnaDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: doudna@berkeley.edu.
University of California, Berkeley · USHoward Hughes Medical Institute · US

Funding

MOLECULAR BIOPHYSICS TRAINING GRANTT32GM008295 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HURLEY, JAMES H, WEMMER, DAVID E · 1989 to 2022
$8.1M
Structure and Function of RNAi-related NucleasesR01GM073794 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI DOUDNA, JENNIFER A · 2005 to 2013
$2.1M
Structure and Function of microRNA Gene Regulation ComplexesF32GM096689 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI WILSON, ROSS C · 2011 to 2013
$148k
Howard Hughes Medical InstituteNIGMS NIH HHS F32 GM096689NIGMS NIH HHS GM073794NIGMS NIH HHS GM096689NIGMS NIH HHS R01 GM073794NIGMS NIH HHS T32 GM008295
6 · The paper itself

Abstract

RNA-mediated gene silencing in human cells requires the accurate generation of ∼22 nt microRNAs (miRNAs) from double-stranded RNA substrates by the endonuclease Dicer. Although the phylogenetically conserved RNA-binding proteins TRBP and PACT are known to contribute to this process, their mode of Dicer binding and their genome-wide effects on miRNA processing have not been determined. We solved the crystal structure of the human Dicer-TRBP interface, revealing the structural basis of the interaction. Interface residues conserved between TRBP and PACT show that the proteins bind to Dicer in a similar manner and by mutual exclusion. Based on the structure, a catalytically active Dicer that cannot bind TRBP or PACT was designed and introduced into Dicer-deficient mammalian cells, revealing selective defects in guide strand selection. These results demonstrate the role of Dicer-associated RNA binding proteins in maintenance of gene silencing fidelity.

Indexed as

AnimalsArgonaute ProteinsCatalytic DomainCells, CulturedCrystallography, X-RayDEAD-box RNA HelicasesGene SilencingHumansMiceMicroRNAsMolecular Sequence DataProtein ConformationRibonuclease IIIRNA-Binding ProteinsSequence AlignmentTrans-Activation Responsive RNA-Binding ProteinArgonaute ProteinsDEAD-box RNA HelicasesDICER1 protein, humanDicer1 protein, mouseMicroRNAsPRKRA protein, humanRibonuclease IIIRNA-Binding ProteinsTrans-Activation Responsive RNA-Binding Protein

Identifiers

PMID25557550
PMCPMC4320653
OpenAlexW2099293835

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.