ArticleAngewandte Chemie (International ed. in English)2015
Visualization and quantification of transmembrane ion transport into giant unilamellar vesicles.
Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Dual-Channel Fluorescence Assays with Supramolecular Host-Dye Reporter Pairs for Membrane Activity Mapping of Peptides.Angewandte Chemie (International ed. in English) · 2026Article
- Incorporation of transmembrane protein antigens into phospholipid bilayers supported on silica microbeads using membrane fusion with budded virions of recombinant baculovirus.Biotechnology letters · 2025Article
- Giant unilamellar vesicles as a model system for studying ion transport.Biophysical reviews · 2025Review
- A Single-Molecule Liposome Assay for Membrane Permeabilization.Angewandte Chemie (International ed. in English) · 2025Article
- Lipid vesicle-based molecular robots.Lab on a chip · 2024Review
- Membrane Models and Experiments Suitable for Studies of the Cholesterol Bilayer Domains.Membranes · 2023Review
- Formation of supramolecular channels by reversible unwinding-rewinding of bis(indole) double helix via ion coordination.Nature communications · 2022Article
- Article
- Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes.Analytical chemistry · 2022Article
- Quantifying proton-induced membrane polarization in single biomimetic giant vesicles.Biophysical journal · 2022Article
- Giant Unilamellar Vesicle Electroformation: What to Use, What to Avoid, and How to Quantify the Results.Membranes · 2021Review
- Supramolecular Chemistry in the Biomembrane.Chembiochem : a European journal of chemical biology · 2020Review
- Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures.ChemistryOpen · 2018Article
- Evaluation of dextran(ethylene glycol) hydrogel films for giant unilamellar lipid vesicle production and their application for the encapsulation of polymersomes.Soft matter · 2017Article
- Targeted anion transporter delivery by coiled-coil driven membrane fusion.Chemical science · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmembrane ion transporters (ionophores) are widely investigated as supramolecular agents with potential for biological activity. Tests are usually performed in synthetic membranes that are assembled into large unilamellar vesicles (LUVs). However transport must be followed through bulk properties of the vesicle suspension, because LUVs are too small for individual study. An alternative approach is described whereby ion transport can be revealed and quantified through direct observation. The method employs giant unilamellar vesicles (GUVs), which are 20-60 μm in diameter and readily imaged by light microscopy. This allows characterization of individual GUVs containing transporter molecules, followed by studies of transport through fluorescence emission from encapsulated indicators. The method provides new levels of certainty and relevance, given that the GUVs are similar in size to living cells. It has been demonstrated using a highly active anion carrier, and should aid the development of compounds for treating channelopathies such as cystic fibrosis.
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