Evidence map›Paper›PMID 25556199›Full record

ArticleThe international journal of neuropsychopharmacology2015

Alcohol Withdrawal-Induced Seizure Susceptibility is Associated with an Upregulation of CaV1.3 Channels in the Rat Inferior Colliculus.

Prosper N'Gouemo, Luli R Akinfiresoye, Joanne S Allard, David M Lovinger

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The international journal of neuropsychopharmacology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. The Inferior Colliculus in Alcoholism and Beyond.Frontiers in systems neuroscience · 2020
    Review
  6. Differential Roles for L-Type Calcium Channel Subtypes in Alcohol Dependence.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2017
    Article
  7. Article
  8. Article
  9. Article
  10. Altered voltage-gated calcium channels in rat inferior colliculus neurons contribute to alcohol withdrawal seizures.European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Prosper N'GouemoGeorgetown University Medical Center, Department of Pediatrics, Washington DC (Drs N'Gouemo and Akinfiesoye); Howard University College of Medicine, Department of Physiology & Biophysics, Washington DC (Dr Allard); Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (Dr Lovinger). pn@georgetown.edu.
Luli R AkinfiresoyeGeorgetown University Medical Center, Department of Pediatrics, Washington DC (Drs N'Gouemo and Akinfiesoye); Howard University College of Medicine, Department of Physiology & Biophysics, Washington DC (Dr Allard); Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (Dr Lovinger).
Joanne S AllardGeorgetown University Medical Center, Department of Pediatrics, Washington DC (Drs N'Gouemo and Akinfiesoye); Howard University College of Medicine, Department of Physiology & Biophysics, Washington DC (Dr Allard); Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (Dr Lovinger).
David M LovingerGeorgetown University Medical Center, Department of Pediatrics, Washington DC (Drs N'Gouemo and Akinfiesoye); Howard University College of Medicine, Department of Physiology & Biophysics, Washington DC (Dr Allard); Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD (Dr Lovinger).
Georgetown University · USGeorgetown University Medical Center · USNational Institute on Alcohol Abuse and Alcoholism · US

Funding

Synaptic Transmission: Modulation, Plasticity And Effects Of Drugs Of AbuseZIAAA000407 · NIAAA · NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM · PI LOVINGER, DAVID M · 2009 to 2025
$29.6M
Corticostriatal mechanisms of action learning and habit formationZIAAA000416 · NIAAA · NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM · PI LOVINGER, DAVID M · 2009 to 2025
$25.2M
Mechanisms of Alcohol Withdrawal Seizures: Role of L-type Ca2+ ChannelsR01AA020073 · NIAAA · GEORGETOWN UNIVERSITY · PI N'GOUEMO, PROSPER · 2011 to 2015
$1.7M
Intramural NIH HHSNIAAA NIH HHS AA020073NIAAA NIH HHS AA020073-03S1NIAAA NIH HHS R01 AA020073
6 · The paper itself

Abstract

backgroundWe previously reported increased current density through L-type voltage-gated Ca(2+) (CaV1) channels in inferior colliculus (IC) neurons during alcohol withdrawal. However, the molecular correlate of this increased CaV1 current is currently unknown.

methodsRats received three daily doses of ethanol every 8 hours for 4 consecutive days; control rats received vehicle. The IC was dissected at various time intervals following alcohol withdrawal, and the mRNA and protein levels of the CaV1.3 and CaV1.2 α1 subunits were measured. In separate experiments, rats were tested for their susceptibility to alcohol withdrawal-induced seizures (AWS) 3, 24, and 48 hours after alcohol withdrawal.

resultsIn the alcohol-treated group, AWS were observed 24 hours after withdrawal; no seizures were observed at 3 or 48 hours. No seizures were observed at any time in the control-treated rats. Compared to control-treated rats, the mRNA level of the CaV1.3 α1 subunit was increased 1.4-fold, 1.9-fold, and 1.3-fold at 3, 24, and 48 hours, respectively. In contrast, the mRNA level of the CaV1.2 α1 subunit increased 1.5-fold and 1.4-fold at 24 and 48 hours, respectively. At 24 hours, Western blot analyses revealed that the levels of the CaV1.3 and CaV1.2 α1 subunits increased by 52% and 32%, respectively, 24 hours after alcohol withdrawal. In contrast, the CaV1.2 and CaV1.3 α1 subunits were not altered at either 3 or 48 hours during alcohol withdrawal.

conclusionsExpression of the CaV1.3 α1 subunit increased in parallel with AWS development, suggesting that altered L-type CaV1.3 channel expression is an important feature of AWS pathogenesis.

Indexed as

Alcohol Withdrawal SeizuresAnimalsCalcium ChannelsCalcium Channels, L-TypeCentral Nervous System DepressantsEthanolInferior ColliculiMaleNeuronsRatsRats, Sprague-DawleyRNA, MessengerTime FactorsUp-RegulationCacna1c protein, ratCacna1d protein, ratCalcium ChannelsCalcium Channels, L-TypeCentral Nervous System DepressantsEthanolRNA, Messengeralcohol withdrawal seizuresCacna1c mRNACacna1d mRNACav1.2 α1 subunitCav1.3 α1 subunit

Identifiers

PMID25556199
PMCPMC4458366
OpenAlexW2139924686

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.