ArticleApplied and environmental microbiology2015
Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 88 citations in OpenAlex.
- A specific tripartite tricarboxylate transporter is involved in alginate utilization byApplied and environmental microbiology · 2026Article
- Characterization of a novel alginate lyase Alg0392 with organic solvent-tolerance from Alteromonas sp. A1-6.Applied microbiology and biotechnology · 2025Article
- Insights into the mechanism of substrate specificity in a novel PL15_3 subfamily oligo-alginate lyase VBAly15A.Applied and environmental microbiology · 2025Article
- Multi-Functional Alginate LyaseMarine drugs · 2025Article
- Alginate-Degrading Modes, Oligosaccharide-Yielding Properties, and Potential Applications of a Novel Bacterial Multifunctional Enzyme, Aly16-1.Microorganisms · 2024Article
- Direct Preparation of Alginate Oligosaccharides from Brown Algae by an Algae-Decomposing Alginate Lyase AlyP18 from the Marine BacteriumMarine drugs · 2024Article
- Genome Analysis of a Potential NovelMarine drugs · 2024Article
- Analysis of unsaturated alginate oligosaccharides using high-performance anion exchange chromatography coupled with mass spectrometry.Analytical and bioanalytical chemistry · 2024Article
- In vitro effects of alginate lyase SG4 + produced by Paenibacillus lautus alone and combined with antibiotics on biofilm formation by mucoid Pseudomonas aeruginosa.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Article
- A bifunctional endolytic alginate lyase with two different lyase catalytic domains fromFrontiers in microbiology · 2024Article
- Genome Sequencing-Based Mining and Characterization of a Novel Alginate Lyase fromMarine drugs · 2023Article
- Cell aggregation is associated with enzyme secretion strategies in marine polysaccharide-degrading bacteria.The ISME journal · 2023Article
- Characterization of Multiple Alginate Lyases in a Highly Efficient Alginate-DegradingApplied and environmental microbiology · 2022Article
- Synergy of the Two Alginate Lyase Domains of a Novel Alginate Lyase fromApplied and environmental microbiology · 2022Article
- Characterization of a Novel Alginate Lyase with Two Alginate Lyase Domains from the Marine BacteriumMarine drugs · 2022Article
- Determination of oligosaccharide product distributions of PL7 alginate lyases by their structural elements.Communications biology · 2022Article
- Cloning and Characterization of a Novel Endo-Type Metal-Independent Alginate Lyase from the Marine BacteriaMarine drugs · 2022Article
- Bacterial growth in multicellular aggregates leads to the emergence of complex life cycles.Current biology : CB · 2022Article
- Biochemical Characterization and Cold-Adaption Mechanism of a PL-17 Family Alginate Lyase Aly23 from Marine BacteriumMarine drugs · 2022Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Alginate lyases are enzymes that degrade alginate through β-elimination of the glycosidic bond into smaller oligomers. We investigated the alginate lyases from Vibrio splendidus 12B01, a marine bacterioplankton species that can grow on alginate as its sole carbon source. We identified, purified, and characterized four polysaccharide lyase family 7 alginates lyases, AlyA, AlyB, AlyD, and AlyE, from V. splendidus 12B01. The four lyases were found to have optimal activity between pH 7.5 and 8.5 and at 20 to 25°C, consistent with their use in a marine environment. AlyA, AlyB, AlyD, and AlyE were found to exhibit a turnover number (kcat) for alginate of 0.60 ± 0.02 s(-1), 3.7 ± 0.3 s(-1), 4.5 ± 0.5 s(-1), and 7.1 ± 0.2 s(-1), respectively. The Km values of AlyA, AlyB, AlyD, and AlyE toward alginate were 36 ± 7 μM, 22 ± 5 μM, 60 ± 2 μM, and 123 ± 6 μM, respectively. AlyA and AlyB were found principally to cleave the β-1,4 bonds between β-d-mannuronate and α-l-guluronate and subunits; AlyD and AlyE were found to principally cleave the α-1,4 bonds involving α-l-guluronate subunits. The four alginate lyases degrade alginate into longer chains of oligomers.
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