ArticleApplied and environmental microbiology2015
Acid-adapted strains of Escherichia coli K-12 obtained by experimental evolution.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Interaction between mutation type and gene pleiotropy drives parallel evolution in the laboratory.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Pooled it
- Using TraDIS as a complementary approach to long term evolution for mapping adaptive mutations in Escherichia coli.PLoS genetics · 2026Article
- Multiple factors based adaptive laboratory evolution strategy enhances DHA production in Aurantiochytrium sp. PKU#Mn16 by rewiring key metabolic pathways.Microbial cell factories · 2025Article
- Effect of conventional grain-fed and grass-fed feeding systems on fecal microbiota and shiga toxin-producing Escherichia coli in beef cattle.BMC microbiology · 2025Article
- Adaptive evolution and reverse engineering to explore the low pH tolerance mechanisms ofApplied and environmental microbiology · 2025Article
- Resistance mechanism of Escherichia coli strains with different ampicillin resistance levels.Applied microbiology and biotechnology · 2024Article
- Adaptive evolution of Salmonella Typhimurium LT2 exposed to carvacrol lacks a uniform pattern.Applied microbiology and biotechnology · 2024Article
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Achieving robust synthetic tolerance in industrialSynthetic and systems biotechnology · 2024Article
- Multitier regulation of theJournal of bacteriology · 2024Article
- Convergent genetic adaptation ofFrontiers in molecular biosciences · 2024Article
- Development of heat-shock resistance inApplied and environmental microbiology · 2023Article
- Enterococcus faecalis OG1RF Evolution at Low pH Selects Fusidate-Sensitive Mutants in Elongation Factor G and at High pH Selects Defects in Phosphate Transport.Applied and environmental microbiology · 2023Article
- A Single Residue within the MCR-1 Protein Confers Anticipatory Resilience.Microbiology spectrum · 2023Article
- Adaptive laboratory evolution ofFrontiers in microbiology · 2023Article
- Rapid Adaptation Often Occurs through Mutations to the Most Highly Conserved Positions of the RNA Polymerase Core Enzyme.Genome biology and evolution · 2022Article
- Synthetic acid stress-tolerance modules improve growth robustness and lysine productivity of industrial Escherichia coli in fermentation at low pH.Microbial cell factories · 2022Article
- Engineering of the Small Noncoding RNA (sRNA) DsrA Together with the sRNA Chaperone Hfq Enhances the Acid Tolerance of Escherichia coli.Applied and environmental microbiology · 2021Article
- Biocide-Induced Emergence of Antibiotic Resistance inFrontiers in microbiology · 2021Article
- Understanding metabolic adaptation by using bacterial laboratory evolution and trans-omics analysis.Biophysical reviews · 2020Review
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Enteric bacteria encounter a wide range of pHs throughout the human intestinal tract. We conducted experimental evolution of Escherichia coli K-12 to isolate clones with increased fitness during growth under acidic conditions (pH 4.5 to 4.8). Twenty-four independent populations of E. coli K-12 W3110 were evolved in LBK medium (10 g/liter tryptone, 5 g/liter yeast extract, 7.45 g/liter KCl) buffered with homopiperazine-N,N'-bis-2-(ethanosulfonic acid) and malate at pH 4.8. At generation 730, the pH was decreased to 4.6 with HCl. By 2,000 generations, all populations had achieved higher endpoint growth than the ancestor at pH 4.6 but not at pH 7.0. All evolving populations showed a progressive loss of activity of lysine decarboxylase (CadA), a major acid stress enzyme. This finding suggests a surprising association between acid adaptation and moderation of an acid stress response. At generation 2,000, eight clones were isolated from four populations, and their genomes were sequenced. Each clone showed between three and eight missense mutations, including one in a subunit of the RNA polymerase holoenzyme (rpoB, rpoC, or rpoD). Missense mutations were found in adiY, the activator of the acid-inducible arginine decarboxylase (adiA), and in gcvP (glycine decarboxylase), a possible acid stress component. For tests of fitness relative to that of the ancestor, lacZ::kan was transduced into each strain. All acid-evolved clones showed a high fitness advantage at pH 4.6. With the cytoplasmic pH depressed by benzoate (at external pH 6.5), acid-evolved clones showed decreased fitness; thus, there was no adaptation to cytoplasmic pH depression. At pH 9.0, acid-evolved clones showed no fitness advantage. Thus, our acid-evolved clones showed a fitness increase specific to low external pH.
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