ArticleGenetics2015
An enhanced gene targeting toolkit for Drosophila: Golic+.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Measuring DNA Double-Strand Break Repair Using the DR-white Assay in Drosophila melanogaster.Journal of visualized experiments : JoVE · 2026Article
- Amyloid precursor protein ortholog Appl acts with Vnd during mushroom body axon growth in Drosophila.Genetics · 2026Article
- Lineage Analysis at Single-Cell Resolution by Twin-Spot MARCM with Lineage-Restricted Drivers.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An approach for quantitative mapping of synaptic periactive zone architecture and organization.Molecular biology of the cell · 2023Article
- Opposing functions for retromer and Rab11 in extracellular vesicle traffic at presynaptic terminals.The Journal of cell biology · 2021Article
- CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila.Nucleic acids research · 2020Article
- Article
- Article
- Short-term activation of the Jun N-terminal kinase pathway in apoptosis-deficient cells of Drosophila induces tumorigenesis.Nature communications · 2018Article
- Engineering the Drosophila Genome for Developmental Biology.Journal of developmental biology · 2017Review
- Review
- Genetic tools to study juvenile hormone action in Drosophila.Scientific reports · 2017Article
- Efficient Screening of CRISPR/Cas9-Induced Events in Drosophila Using a Co-CRISPR Strategy.G3 (Bethesda, Md.) · 2017Article
- Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Coconversion.G3 (Bethesda, Md.) · 2016Article
- Building a ladder to Hershey Heaven.eLife · 2016Article
- Tissue-specific tagging of endogenous loci in Drosophila melanogaster.Biology open · 2015Article
- Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research.G3 (Bethesda, Md.) · 2015Article
- Genome engineering: Drosophila melanogaster and beyond.Wiley interdisciplinary reviews. Developmental biologyReview
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Ends-out gene targeting allows seamless replacement of endogenous genes with engineered DNA fragments by homologous recombination, thus creating designer "genes" in the endogenous locus. Conventional gene targeting in Drosophila involves targeting with the preintegrated donor DNA in the larval primordial germ cells. Here we report G: ene targeting during O: ogenesis with L: ethality I: nhibitor and C: RISPR/Cas (Golic+), which improves on all major steps in such transgene-based gene targeting systems. First, donor DNA is integrated into precharacterized attP sites for efficient flip-out. Second, FLP, I-SceI, and Cas9 are specifically expressed in cystoblasts, which arise continuously from female germline stem cells, thereby providing a continual source of independent targeting events in each offspring. Third, a repressor-based lethality selection is implemented to facilitate screening for correct targeting events. Altogether, Golic+ realizes high-efficiency ends-out gene targeting in ovarian cystoblasts, which can be readily scaled up to achieve high-throughput genome editing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.