Evidence map›Paper›PMID 25554787›Full record

ArticleScience (New York, N.Y.)2015

Protein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains.

Peter S Shen, Joseph Park, Yidan Qin, Xueming Li, Krishna Parsawar, Matthew H Larson, James Cox, Yifan Cheng, Alan M Lambowitz, Jonathan S Weissman and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 187 papers.

0numbers the graph read from it
0cells of the map it votes in
187citing papers in PubMed
14.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

187 citing papers in PubMed, 307 citations in OpenAlex.

  1. Translation factors that keep the ribosome Pro-active.Microbiology and molecular biology reviews : MMBR · 2026
    Review
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Ribosome-associated quality control and related mechanisms.Nature structural & molecular biology · 2026
    Review
  8. Article
  9. Quality control and signaling pathways at stalled ribosomes.Experimental & molecular medicine · 2026
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Plant MutS2 proteins function in plastid ribosome quality control.bioRxiv : the preprint server for biology · 2025
    Article
  19. Review
  20. Article

127 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Peter S ShenDepartment of Biochemistry, University of Utah, UT 84112, USA.
Joseph ParkDepartment of Biochemistry, Stanford University, Palo Alto, CA 94305, USA.
Yidan QinInstitute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA. Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.
Xueming LiDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Krishna ParsawarMass Spectrometry and Proteomics Core Facility, University of Utah, UT 84112, USA.
Matthew H LarsonDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA. Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA. California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158, USA. Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA.
James CoxDepartment of Biochemistry, University of Utah, UT 84112, USA. Mass Spectrometry and Proteomics Core Facility, University of Utah, UT 84112, USA.
Yifan ChengDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Alan M LambowitzInstitute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA. Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA.
Jonathan S WeissmanDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA. Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA. California Institute for Quantitative Biomedical Research, University of California, San Francisco, San Francisco, CA 94158, USA. Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA. jonathan.weissman@ucsf.edu onn@stanford.edu adam.frost@ucsf.edu.
Onn BrandmanDepartment of Biochemistry, Stanford University, Palo Alto, CA 94305, USA. jonathan.weissman@ucsf.edu onn@stanford.edu adam.frost@ucsf.edu.
Adam FrostDepartment of Biochemistry, University of Utah, UT 84112, USA. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA. jonathan.weissman@ucsf.edu onn@stanford.edu adam.frost@ucsf.edu.
University of California, San Francisco · USThe University of Texas at Austin · USPalo Alto University · USQB3 · USStanford University · US

Funding

PROJECT 3P50GM102706 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CATE, JAMIE H · 2012 to 2016
$9.5M
RETROPLASMID AND GROUP II INTRON REVERSE TRANSCRIPTASESR01GM037949 · NIGMS · UNIVERSITY OF TEXAS AUSTIN · PI LAMBOWITZ, ALAN M. · 1986 to 2019
$8.6M
RNA SPLICING IN NEUROSPORA MITOCHONDRIAR01GM037951 · NIGMS · UNIVERSITY OF TEXAS AUSTIN · PI LAMBOWITZ, ALAN M. · 1986 to 2016
$6.6M
Structural Basis of Protein HomeostasisU01GM098254 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AGARD, DAVID A., FRYDMAN, JUDITH · 2012 to 2014
$3.2M
Request for Support to Purchase Automated MicroscopeR01GM115968 · NIGMS · STANFORD UNIVERSITY · PI BRANDMAN, ONN · 2015 to 2023
$2.9M
Toward Atomic Resolution of Membranes and Membrane-Associated MachinesDP2GM110772 · NIGMS · UNIVERSITY OF UTAH · PI FROST, ADAM · 2013 to 2013
$2.2M
INVOLVEMENT OF PROTEINS IN SPLICING GROUP I INTRONSR37GM037951 · NIGMS · UNIVERSITY OF TEXAS AUSTIN · PI LAMBOWITZ, ALAN M. · 1993 to 2002
$1.2M
Howard Hughes Medical InstituteNCCDPHP CDC HHS 1DP2GM110772-01NIGMS NIH HHS DP2 GM110772NIGMS NIH HHS GM37949NIGMS NIH HHS GM37951NIGMS NIH HHS P50 GM102706NIGMS NIH HHS R01 GM037949NIGMS NIH HHS R01 GM037951NIGMS NIH HHS R01 GM115968NIGMS NIH HHS R37 GM037951NIGMS NIH HHS U01 GM098254
6 · The paper itself

Abstract

In Eukarya, stalled translation induces 40S dissociation and recruitment of the ribosome quality control complex (RQC) to the 60S subunit, which mediates nascent chain degradation. Here we report cryo-electron microscopy structures revealing that the RQC components Rqc2p (YPL009C/Tae2) and Ltn1p (YMR247C/Rkr1) bind to the 60S subunit at sites exposed after 40S dissociation, placing the Ltn1p RING (Really Interesting New Gene) domain near the exit channel and Rqc2p over the P-site transfer RNA (tRNA). We further demonstrate that Rqc2p recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits. Our work uncovers an unexpected mechanism of protein synthesis, in which a protein--not an mRNA--determines tRNA recruitment and the tagging of nascent chains with carboxy-terminal Ala and Thr extensions ("CAT tails").

Indexed as

Peptide Biosynthesis, Nucleic Acid-IndependentCryoelectron MicroscopyNucleic Acid ConformationProtein ConformationRibosome Subunits, Large, EukaryoticRNA-Binding ProteinsRNA, MessengerRNA, Transfer, AlaRNA, Transfer, ThrSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsUbiquitin-Protein LigasesLtn1 protein, S cerevisiaeRkr1 protein, S cerevisiaeRNA-Binding ProteinsRNA, MessengerRNA, Transfer, AlaRNA, Transfer, ThrSaccharomyces cerevisiae ProteinsTae2 protein, S cerevisiaeUbiquitin-Protein Ligases

Identifiers

PMID25554787
PMCPMC4451101
OpenAlexW1985387528

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.