Evidence map›Paper›PMID 25554747›Full record

ArticleJournal of otology & rhinology2014

Volume Expansion of Tissue Engineered Human Nasal Septal Cartilage.

Marsha S Reuther, Kristen K Briggs, Monica K Neuman, Koichi Masuda, Robert L Sah, Deborah Watson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of otology & rhinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Dorsal Augmentation Rhinoplasty by Cartilage Allograft.Journal of cosmetic dermatology · 2025
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Marsha S ReutherDivision of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, USA ; Head and Neck Surgery Section, VA San Diego Healthcare System, San Diego, California, USA.
Kristen K BriggsDepartment of Bioengineering, University of California, San Diego, La Jolla, California, USA ; Research Service, VA San Diego Healthcare System, San Diego, California, USA.
Monica K NeumanCreighton University School of Medicine, Omaha, Nebraska, USA.
Koichi MasudaDepartment of Orthopedic Surgery, University of California, San Diego, La Jolla, California, USA.
Robert L SahDepartment of Bioengineering, University of California, San Diego, La Jolla, California, USA.
Deborah WatsonDivision of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, USA ; Head and Neck Surgery Section, VA San Diego Healthcare System, San Diego, California, USA.
VA San Diego Healthcare System · US

Funding

TISSUE ACQUISITION, MORPHOLOGY, AND CELL CULTUREP01AG007996 · NIA · UNIVERSITY OF CALIFORNIA SAN DIEGO · PI TERKELTAUB, ROBERT A. · 1989 to 2016
$26.0M
Developing Articular Cartilage: Function &RemodelingR01AR044058 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SAH, ROBERT L · 2000 to 2010
$3.3M
UCSD Medical Student Summer Aging Research TrainingT35AG026757 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Benjamin Hyun Han, Ellen Eun-Ok Lee · 2005 to 2026
$2.2M
OTOLARYNGOLOGY TRAINING IN IMMUN., VIROL. &MOLEC. BIOL.T32DC000028 · NIDCD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RYAN, ALLEN F. · 1989 to 2020
$2.1M
LANGUAGE-BASED MEMORY AND SPATIAL MEMORY IN THE DEAFF32DC000128 · NIDCD · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI WILSON, MARGARET L · 1993 to 1996
–
NIAMS NIH HHS R01 AR044058NIA NIH HHS P01 AG007996NIA NIH HHS T35 AG026757NIDCD NIH HHS F32 DC000128NIDCD NIH HHS T32 DC000028
6 · The paper itself

Abstract

importanceCartilaginous craniofacial defects range in size and autologous cartilaginous tissue is preferred for repair of these defects. Therefore, it is important to have the ability to produce large size cartilaginous constructs for repair of cartilaginous abnormalities.

objectivesTo produce autologous human septal neocartilage constructs substantially larger in size than previously produced constructsTo demonstrate that volume expanded neocartilage constructs possess comparable histological and biochemical properties to standard size constructsTo show that volume expanded neocartilage constructs retain similar biomechanical properties to standard size constructs.

designProspective, basic science.

settingLaboratory.

participantsThe study used remnant human septal specimens removed during routine surgery at the University of California, San Diego Medical Center or San Diego Veterans Affairs Medical Center. Cartilage from a total of 8 donors was collected. MAIN OUTCOMES MEASURED: Human septal chondrocytes from 8 donors were used to create 12mm and 24mm neocartilage constructs. These were cultured for a total of 10 weeks. Photo documentation, histological, biochemical, and biomechanical properties were measured and compared.

resultsThe 24mm diameter constructs were qualitatively similar to the 12mm constructs. They possessed adequate strength and durability to be manually manipulated. Histological analysis of the constructs demonstrated similar staining patterns in standard and volume expanded constructs. Proliferation, as measured by DNA content, was similar in 24mm and 12mm constructs. Additionally, glycosaminoglycan (GAG) and total collagen content did not significantly differ between the two construct sizes. Biomechanical analysis of the 24mm and 12mm constructs demonstrated comparable compressive and tensile properties. CONCLUSION AND RELEVANCE: Volume expanded human septal neocartilage constructs are qualitatively and histologically similar to standard 12mm constructs. Biochemical and biomechanical analysis of the constructs demonstrated equivalent properties. This study shows that modification of existing protocols is not required to successfully produce neocartilage constructs in larger sizes for reconstruction of more substantial craniofacial defects. LEVEL OF EVIDENCE: NA.

Indexed as

Cartilage constructCartilage tissue engineeringHuman septal cartilage

Identifiers

PMID25554747
PMCPMC4279869
OpenAlexW2033185641

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.