Evidence map›Paper›PMID 25554740›Full record

ArticleGenomics data2015

Locus-specific DNA methylation analysis of retrotransposons in ES, somatic and cancer cells using High-Throughput Targeted Repeat Element Bisulfite Sequencing.

Arundhati Bakshi, Muhammad B Ekram, Joomyeong Kim

Abstract read
In one paragraph

Article in Genomics data, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Epigenetic mechanism controlsClinical interventions in aging · 2019
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Arundhati BakshiDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Muhammad B EkramDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Joomyeong KimDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.

Funding

Imprinting studies of a paternally expressed Usp29 geneR01GM066225 · NIGMS · UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB · PI KIM, JOOMYEONG · 2004 to 2013
$2.6M
Aebp2 as an epigenetic regulator for neural crest cellR01GM097074 · NIGMS · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI KIM, JOOMYEONG · 2013 to 2016
$1.1M
Retrotransposon as a major source to epigenetic variations in the human genomeR15ES019118 · NIEHS · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI KIM, JOOMYEONG · 2010 to 2010
$436k
NIEHS NIH HHS R15 ES019118NIGMS NIH HHS R01 GM066225NIGMS NIH HHS R01 GM097074
6 · The paper itself

Abstract

DNA methylation is a major epigenetic mark associated with multiple aspects of retrotransposons within the mammalian genome. In order to study DNA methylation of a large number of retrotransposons on an individual-locus basis, we have developed a new protocol termed High-Throughput Targeted Repeat Element Bisulfite Sequencing (HT-TREBS) (Ekram and Kim, 2014 [1]). We have used this technique to characterize the locus-specific patterns of DNA methylation of 4799 members of the mouse IAP LTR (Intracisternal A Particle Long Terminal Repeat) retrotransposon family in embryonic stem, somatic and Neuro2A cells (Bakshi and Kim, 2014 [2]). Here we describe in detail the sample preparation and bioinformatics analyses used for these studies. The somatic cell data may be accessed under GEO accession number GSE49222. The ES and Neuro2A data are deposited under GEO accession number GSE60007.

Indexed as

DNA methylationendogenous retrovirusHT-TREBSIAP LTRretrotransposons

Identifiers

PMID25554740
PMCPMC4278373

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.