ArticleJournal of neuroscience methods2015
A novel method to study cerebrospinal fluid dynamics in rats.
Article in Journal of neuroscience methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 76 citations in OpenAlex.
- CSF hypersecretion versus impaired CSF absorption in posthemorrhagic hydrocephalus: a systematic review.Acta neurochirurgica · 2023Pooled it
- Estimation of metabolic water production in human and rat brain and spinal cord.Fluids and barriers of the CNS · 2026Article
- Transient acute neuronal activation response caused by high concentrations of oligonucleotides in the cerebral spinal fluid.Nucleic acids research · 2026Article
- Indirect Deuterium Displacement Exchange Imaging for Noninvasive High-Resolution CSF Production Mapping.bioRxiv : the preprint server for biology · 2025Article
- Effect of intracerebral infusion rate on optic nerve tissue pressure and diurnal intracranial pressure in rats.Fluids and barriers of the CNS · 2025Article
- Choroid plexus-mediated CSF secretion remains stable in aging rats via high and age-resistant metabolic activity.Nature communications · 2025Article
- Perivascular cerebrospinal fluid inflow matches interstitial fluid efflux in anesthetized rats.iScience · 2025Article
- The Cerebrospinal Fluid Secretion Rate Increases in Awake and Freely Moving Rats but Differs With Experimental Methodology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- PTEN mutations impair CSF dynamics and cortical networks by dysregulating periventricular neural progenitors.Nature neuroscience · 2025Article
- Communicating hydrocephalus following stereotactic radiosurgery for periventricular meningiomas: illustrative cases.Journal of neurosurgery. Case lessons · 2025Article
- Article
- Cerebrospinal fluid pressure dynamics as a biomechanical marker for quantification of spinal cord compression: Conceptual framework and systematic review of clinical trials.Brain & spine · 2025Review
- Relaxation-exchange magnetic resonance imaging (REXI): a non-invasive imaging method for evaluating trans-barrier water exchange in the choroid plexus.Fluids and barriers of the CNS · 2024Article
- Spatial Intracranial Pressure Fields Driven by Blast Overpressure in Rats.Annals of biomedical engineering · 2024Article
- CSF hyperdynamics in rats mimicking the obesity and androgen excess characteristic of patients with idiopathic intracranial hypertension.Fluids and barriers of the CNS · 2024Article
- The relationship between myodural bridge, atrophy and hyperplasia of the suboccipital musculature, and cerebrospinal fluid dynamics.Scientific reports · 2023Article
- Nocturnal increase in cerebrospinal fluid secretion as a circadian regulator of intracranial pressure.Fluids and barriers of the CNS · 2023Article
- Are standing osmotic gradients the main driver of cerebrospinal fluid production? A computational analysis.Fluids and barriers of the CNS · 2023Article
- Article
- Activation of the RARα Attenuated CSF Hypersecretion to Inhibit Hydrocephalus Development via Regulating the MAFB/MSR1 Pathway.International journal of molecular sciences · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCerebrospinal fluid (CSF) flow dynamics play critical roles in both the immature and adult brain, with implications for neurodevelopment and disease processes such as hydrocephalus and neurodegeneration. Remarkably, the only reported method to date for measuring CSF formation in laboratory rats is the indirect tracer dilution method (a.k.a., ventriculocisternal perfusion), which has limitations. NEW
methodAnesthetized rats were mounted in a stereotaxic apparatus, both lateral ventricles were cannulated, and the Sylvian aqueduct was occluded. Fluid exited one ventricle at a rate equal to the rate of CSF formation plus the rate of infusion (if any) into the contralateral ventricle. Pharmacological agents infused at a constant known rate into the contralateral ventricle were tested for their effect on CSF formation in real-time.
resultsThe measured rate of CSF formation was increased by blockade of the Sylvian aqueduct but was not changed by increasing the outflow pressure (0-3cm of H2O). In male Wistar rats, CSF formation was age-dependent: 0.39±0.06, 0.74±0.05, 1.02±0.04 and 1.40±0.06μL/min at 8, 9, 10 and 12 weeks, respectively. CSF formation was reduced 57% by intraventricular infusion of the carbonic anhydrase inhibitor, acetazolamide. COMPARISON WITH EXISTING
methodsTracer dilution methods do not permit ongoing real-time determination of the rate of CSF formation, are not readily amenable to pharmacological manipulations, and require critical assumptions. Direct measurement of CSF formation overcomes these limitations.
conclusionsDirect measurement of CSF formation in rats is feasible. Our method should prove useful for studying CSF dynamics in normal physiology and disease models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.