Evidence map›Paper›PMID 25554414›Full record

ArticleJournal of neuroscience methods2015

Spatial characterization of a multifunctional pipette for drug delivery in hippocampal brain slices.

Aikeremu Ahemaiti, Holger Wigström, Alar Ainla, Gavin D M Jeffries, Owe Orwar, Aldo Jesorka, Kent Jardemark

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Aikeremu AhemaitiDepartment of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden.
Holger WigströmInstitute of Neuroscience and Physiology, The Sahlgrenska Academy at Göteborg University, Box 430, SE-40530 Göteborg, Sweden.
Alar AinlaDepartment of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden.
Gavin D M JeffriesDepartment of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden.
Owe OrwarDepartment of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden.
Aldo JesorkaDepartment of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-41296 Göteborg, Sweden.
Kent JardemarkDepartment of Physiology and Pharmacology, Karolinska Institutet, SE-17177 Stockholm, Sweden. Electronic address: Kent.Jardemark@ki.se.
Chalmers University of Technology · SEKarolinska Institutet · SEUniversity of Gothenburg · SE

Funding

Single Cell ElectroporationR01GM066018 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WEBER, STEPHEN G. · 2003 to 2014
$3.1M
NIGMS NIH HHS GM R01-066018NIGMS NIH HHS R01 GM066018
6 · The paper itself

Abstract

backgroundAmong the various fluidic control technologies, microfluidic devices are becoming powerful tools for pharmacological studies using brain slices, since these devices overcome traditional limitations of conventional submerged slice chambers, leading to better spatiotemporal control over delivery of drugs to specific regions in the slices. However, microfluidic devices are not yet fully optimized for such studies. NEW

methodWe have recently developed a multifunctional pipette (MFP), a free standing hydrodynamically confined microfluidic device, which provides improved spatiotemporal control over drug delivery to biological tissues.

resultsWe demonstrate herein the ability of the MFP to selectively perfuse one dendritic layer in the CA1 region of hippocampus with CNQX, an AMPA receptor antagonist, while not affecting the other layers in this region. Our experiments also illustrate the essential role of hydrodynamic confinement in sharpening the spatial selectivity in brain slice experiments. Concentration-response measurements revealed that the ability of the MFP to control local drug concentration is comparable with that of whole slice perfusion, while in comparison the required amounts of active compounds can be reduced by several orders of magnitude. COMPARISON WITH EXISTING

methodThe multifunctional pipette is applied with an angle, which, compared to other hydrodynamically confined microfluidic devices, provides more accessible space for other probing and imaging techniques.

conclusionsUsing the MFP it will be possible to study selected regions of brain slices, integrated with various imaging and probing techniques, without affecting the other parts of the slices.

Indexed as

AnimalsDose-Response Relationship, DrugDrug Delivery SystemsHippocampusMicrofluidic Analytical TechniquesOrgan Culture TechniquesPharmaceutical PreparationsRatsRats, Sprague-DawleyPharmaceutical PreparationsBrain slicesElectrophysiological techniquesGlutamateHippocampusMicrofluidic technologyMultifunctional pipettePerfusion

Identifiers

PMID25554414
PMCPMC4324357
OpenAlexW2001583975

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.