Evidence map›Paper›PMID 25553616›Full record

ArticleBrain research2015

Functional alpha7 nicotinic receptors are expressed on immature granule cells of the postnatal dentate gyrus.

Danielle John, Irina Shelukhina, Yuchio Yanagawa, Jim Deuchars, Zaineb Henderson

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Danielle JohnSchool of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. Electronic address: djohn@ucsd.edu.
Irina ShelukhinaDepartment of Molecular Basis of Neurosignaling, Laboratory of Molecular Toxinology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow V-437, Russia.
Yuchio YanagawaDepartment of Genetic and Behavioural Neuroscience, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan; Japan Science and Technology Agency, CREST, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan.
Jim DeucharsSchool of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
Zaineb HendersonSchool of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK.
University of Leeds · GBInstitute of Bioorganic Chemistry · RUJapan Science and Technology Agency · JP

Funding

Biotechnology and Biological Sciences Research CouncilMedical Research Council G0500823
6 · The paper itself

Abstract

Neurogenesis occurs throughout life in the subgranular zone of the dentate gyrus, and postnatal-born granule cells migrate into the granule cell layer and extend axons to their target areas. The α7*nicotinic receptor has been implicated in neuronal maturation during development of the brain and is abundant in interneurons of the hippocampal formation of the adult brain. Signalling through these same receptors is believed also to promote maturation and integration of adult-born granule cells in the hippocampal formation. We therefore aimed to determine whether functional α7*nicotinic receptors are expressed in developing granule cells of the postnatal dentate gyrus. For these experiments we used 2-3 week-old Wistar rats, and 2-9 week old transgenic mice in which GABAergic interneurons were marked by expression of green fluorescent protein. Immunohistochemistry indicated the presence of α7*nicotinic receptor subunits around granule cells close around the subgranular zone which correlated with the distribution of developmental markers for immature granule cells. Whole-cell patch clamp recording showed that a proportion of granule cells responded to puffed ACh in the presence of atropine, and that these cells possessed electrophysiological properties found in immature granule cells. The nicotinic responses were potentiated by an allosteric α7*nicotinic receptor modulator, which were blocked by a specific α7*nicotinic receptor antagonist and were not affected by ionotropic glutamate or GABA receptor antagonists. These results suggest the presence of functional somato-dendritic α7*nicotinic receptors on immature granule cells of the postnatal dentate gyrus, consistent with studies implicating α7*nicotinic receptors in dendritic maturation of dentate gyrus neurons in adult brain.

Indexed as

Acetylcholinealpha7 Nicotinic Acetylcholine ReceptorAnimalsAntibodiesCholinergic AgonistsDentate GyrusGABAergic NeuronsInterneuronsMembrane PotentialsMiceMice, TransgenicRatsRats, WistarAcetylcholinealpha7 Nicotinic Acetylcholine ReceptorAntibodiesCholinergic AgonistsAlpha 7 nicotinic acetylcholine receptorsDentate gyrusNeurogenesis

Identifiers

PMID25553616
PMCPMC4350854
OpenAlexW2000285037

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.