ArticleAnnals of laboratory medicine2015
Comparison of quantitation of cytomegalovirus DNA by real-time PCR in whole blood with the cytomegalovirus antigenemia assay.
Article in Annals of laboratory medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Transition from Cytomegalovirus Antigenemia Assay and Whole-Blood Quantitative PCR to Automated Plasma Quantitative PCR in Solid Organ Transplant Recipients: Correlation of Viral Loads and Impact on Treatment Eligibility Based on a Single-Center Retrospective Study.Annals of laboratory medicine · 2026Article
- Diagnostic performance of noninvasive tests for cytomegalovirus ileocolitis: a systematic review and meta-analysis.Intestinal research · 2025Article
- Article
- Severe CMV pneumonitis and the resulting ARDS in a 28-year-old pregnant woman: a case report.BMC infectious diseases · 2023Article
- Laboratory diagnostic testing for cytomegalovirus infection in solid organ transplant patients.Korean journal of transplantation · 2022Review
- Plasma cytomegalovirus DNA load predicts outcomes in liver transplant recipients.Immunity, inflammation and disease · 2021Article
- Cytomegalovirus shedding in seropositive healthy women of reproductive age in Tianjin, China.Epidemiology and infection · 2020Article
- Relationship of Circulating Cytomegalovirus Levels Obtained Through Antigenemia Testing and Quantitative PCR Differs Between Children and Adults.Annals of laboratory medicine · 2020Article
- Colon perforation due to antigenemia-negative cytomegalovirus gastroenteritis after liver transplantation: A case report and review of literature.World journal of gastroenterology · 2019Review
- Factors associated with cytomegalovirus infection in children undergoing allogeneic hematopoietic stem-cell transplantation.Medicine · 2019Article
- Intestinal perforation due to hemorrhagic Cytomegalovirus enteritis in a patient with severe uncontrolled lupus nephritis: a case and review of the literature.Rheumatology international · 2017Review
- Clinical validation of an in-house quantitative real time PCR assay for cytomegalovirus infection using the 1st WHO International Standard in kidney transplant patients.Jornal brasileiro de nefrologiaArticle
- Cost minimization analysis of an in-house molecular test for cytomegalovirus in relation to a commercial molecular system.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitation of cytomegalovirus (CMV) DNA using real-time PCR has been utilized for monitoring CMV infection. However, the CMV antigenemia assay is still the 'gold standard' assay. There are only a few studies in Korea that compared the efficacy of use of real-time PCR for quantitation of CMV DNA in whole blood with the antigenemia assay, and most of these studies have been limited to transplant recipients.
method479 whole blood samples from 79 patients, falling under different disease groups, were tested by real-time CMV DNA PCR using the Q-CMV real-time complete kit (Nanogen Advanced Diagnostic S.r.L., Italy) and CMV antigenemia assay (CINA Kit, ArgeneBiosoft, France), and the results were compared. Repeatedly tested patients were selected and their charts were reviewed for ganciclovir therapy.
resultsThe concordance rate of the two assays was 86.4% (Cohen's kappa coefficient value=0.659). Quantitative correlation between the two assays was a moderate (r=0.5504, P<0.0001). Among 20 patients tested repeatedly with the two assays, 13 patients were transplant recipients and treated with ganciclovir. Before treatment, CMV was detected earlier by real-time CMV DNA PCR than the antigenemia assay, with a median difference of 8 days. After treatment, the antigenemia assay achieved negative results earlier than real-time CMV DNA PCR with a median difference of 10.5 days.
conclusionsQ-CMV real-time complete kit is a useful tool for early detection of CMV infection in whole blood samples in transplant recipients.
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