ArticleBiomicrofluidics2014
Microfluidic impedance cytometry of tumour cells in blood.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 71 citations in OpenAlex.
- Code-multiplexed multi-frequency impedance cytometry with a unified deep-unfolding network.Microsystems & nanoengineering · 2026Article
- A Review on Microfluidics-Based Impedance Biosensors.Biosensors · 2023Review
- Label-Free Microfluidic Impedance Cytometry for Acrosome Integrity Assessment of Boar Spermatozoa.Biosensors · 2022Article
- Microfluidics for the Isolation and Detection of Circulating Tumor Cells.Advances in experimental medicine and biology · 2022Article
- Recent Advances in Electrical Impedance Sensing Technology for Single-Cell Analysis.Biosensors · 2021Review
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- A fast impedance-based antimicrobial susceptibility test.Nature communications · 2020Article
- Electrophysiology-based stratification of pancreatic tumorigenicity by label-free single-cell impedance cytometry.Analytica chimica acta · 2020Article
- Post-enrichment circulating tumor cell detection and enumeration via deformability impedance cytometry.Biosensors & bioelectronics · 2020Article
- Magnetic nanoparticles for smart electrochemical immunoassays: a review on recent developments.Mikrochimica acta · 2019Review
- Dielectric characterization ofJournal of the Royal Society, Interface · 2018Article
- Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting.Journal of the Royal Society, Interface · 2017Article
- Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry.Scientific reports · 2017Article
- Using binary optical elements (BOEs) to generate rectangular spots for illumination in micro flow cytometer.Biomicrofluidics · 2016Article
- Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement.Biomicrofluidics · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dielectric properties of tumour cells are known to differ from normal blood cells, and this difference can be exploited for label-free separation of cells. Conventional measurement techniques are slow and cannot identify rare circulating tumour cells (CTCs) in a realistic timeframe. We use high throughput single cell microfluidic impedance cytometry to measure the dielectric properties of the MCF7 tumour cell line (representative of CTCs), both as pure populations and mixed with whole blood. The data show that the MCF7 cells have a large membrane capacitance and size, enabling clear discrimination from all other leukocytes. Impedance analysis is used to follow changes in cell viability when cells are kept in suspension, a process which can be understood from modelling time-dependent changes in the dielectric properties (predominantly membrane conductivity) of the cells. Impedance cytometry is used to enumerate low numbers of MCF7 cells spiked into whole blood. Chemical lysis is commonly used to remove the abundant erythrocytes, and it is shown that this process does not alter the MCF7 cell count or change their dielectric properties. Combining impedance cytometry with magnetic bead based antibody enrichment enables MCF7 cells to be detected down to 100 MCF7 cells in 1 ml whole blood, a log 3.5 enrichment and a mean recovery of 92%. Microfluidic impedance cytometry could be easily integrated within complex cell separation systems for identification and enumeration of specific cell types, providing a fast in-line single cell characterisation method.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.