ArticleBiomicrofluidics2014
Stretching of DNA confined in nanochannels with charged walls.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- DsbA is a redox-switchable mechanical chaperone.Chemical science · 2021Article
- Nanofluidic-Based Accumulation of Antigens for Miniaturized Immunoassay.Sensors (Basel, Switzerland) · 2020Article
- Increased Flexibility in Lab-on-Chip Design with a Polymer Patchwork Approach.Nanomaterials (Basel, Switzerland) · 2019Article
- Dynamic simulations show repeated narrowing maximizes DNA linearization in elastomeric nanochannels.Biomicrofluidics · 2016Article
- Modeling the relaxation of internal DNA segments during genome mapping in nanochannels.Biomicrofluidics · 2016Article
- Finite-size corrections for confined polymers in the extended de Gennes regime.Physical review. E, Statistical, nonlinear, and soft matter physics · 2015Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is currently a growing interest in control of stretching of DNA inside nanoconfined regions due to the possibility to analyze and manipulate single biomolecules for applications such as DNA mapping and barcoding, which are based on stretching the DNA in a linear fashion. In the present work, we couple Finite Element Methods and Monte Carlo simulations in order to study the conformation of DNA molecules confined in nanofluidic channels with neutral and charged walls. We find that the electrostatic forces become more and more important when lowering the ionic strength of the solution. The influence of the nanochannel cross section geometry is also studied by evaluating the DNA elongation in square, rectangular, and triangular channels. We demonstrate that coupling electrostatically interacting walls with a triangular geometry is an efficient way to stretch DNA molecules at the scale of hundreds of nanometers. The paper reports experimental observations of λ-DNA molecules in poly(dimethylsiloxane) nanochannels filled with solutions of different ionic strength. The results are in good agreement with the theoretical predictions, confirming the crucial role of the electrostatic repulsion of the constraining walls on the molecule stretching.
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Registered trials
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