ArticleBiomicrofluidics2014
Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Glioma chemotherapeutic resistance is tied to membrane electrophysiological properties and glycosylation.Bioengineering & translational medicine · 2026Article
- Electrical Impedance Spectroscopy as a Tool to Detect the Epithelial to Mesenchymal Transition in Prostate Cancer Cells.Biosensors · 2024Article
- Phenotypic Characterization of 2D and 3D Prostate Cancer Cell Systems Using Electrical Impedance Spectroscopy.Biosensors · 2023Article
- A High-Throughput Microfluidic Cell Sorter Using a Three-Dimensional Coupled Hydrodynamic-Dielectrophoretic Pre-Focusing Module.Micromachines · 2023Article
- Dielectrophoretic Characterization of Tenogenically Differentiating Mesenchymal Stem Cells.Biosensors · 2021Article
- New method for arbuscular mycorrhizal fungus spore separation using a microfluidic device based on manual temporary flow diversion.Mycorrhiza · 2020Article
- Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer.Analytical and bioanalytical chemistry · 2020Article
- Label-free enrichment of fate-biased human neural stem and progenitor cells.Biosensors & bioelectronics · 2020Article
- Growth and Spatial Control of Murine Neural Stem Cells on Reflectin Films.ACS biomaterials science & engineering · 2020Article
- High-throughput continuous dielectrophoretic separation of neural stem cells.Biomicrofluidics · 2019Article
- Identification of neural stem and progenitor cell subpopulations using DC insulator-based dielectrophoresis.The Analyst · 2019Article
- Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells.Stem cell reports · 2018Article
- It's Electric: When Technology Gives a Boost to Stem Cell Science.Current stem cell reports · 2018Article
- Biomanufacturing for clinically advanced cell therapies.Nature biomedical engineering · 2018Review
- Separation of neural stem cells by whole cell membrane capacitance using dielectrophoresis.Methods (San Diego, Calif.) · 2018Review
- Dielectrophoresis-based microfluidic platforms for cancer diagnostics.Biomicrofluidics · 2018Review
- High-throughput, low-loss, low-cost, and label-free cell separation using electrophysiology-activated cell enrichment.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a label-free manner and its biological safety. However, DEP separation devices have suffered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We developed a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of magnitude. This advancement, coupled with data showing the DEP-sorted cells retain their enrichment and differentiation capacity when expanded in culture for periods of up to 2 weeks, provides sufficient throughput and cell numbers to enable a wider variety of experiments with enriched stem and progenitor cell populations. Furthermore, the sorting devices presented here provide ease of setup and operation, a simple fabrication process, and a low associated cost to use that makes them more amenable for use in common biological research laboratories. To our knowledge, this work represents the first to enrich stem cells and expand them in culture to generate transplantation-scale numbers of differentiation-competent cells using DEP.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.