ArticleBiomicrofluidics2014
A microfluidic device to apply shear stresses to polarizing ciliated airway epithelium using air flow.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Development of a Widely Accessible, Advanced Large-Scale Microfluidic Airway-on-Chip.Bioengineering (Basel, Switzerland) · 2025Article
- Inhalable Mucociliary-On-Chip System Revealing Pulmonary Clearance Dynamics in Nanodrug Delivery.ACS nano · 2025Article
- The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 2-Advanced Microfluidic Nasal In Vitro Model for Drug Absorption Testing.Pharmaceutics · 2023Article
- Bronchial epithelial and airway smooth muscle cell interactions in health and disease.Heliyon · 2023Review
- Polymeric and biological membranes for organ-on-a-chip devices.Microsystems & nanoengineering · 2023Review
- PDMS Organ-On-Chip Design and Fabrication: Strategies for Improving Fluidic Integration and Chip Robustness of Rapidly Prototyped Microfluidic In Vitro Models.Micromachines · 2022Article
- 3D printed transwell-integrated nose-on-chip model to evaluate effects of air flow-induced mechanical stresses on mucous secretion.Biomedical microdevices · 2022Article
- Computational Fluid Dynamic Modeling Reveals Nonlinear Airway Stress during Trachea Development.The Journal of pediatrics · 2021Article
- Review
- Cilia density and flow velocity affect alignment of motile cilia from brain cells.The Journal of experimental biology · 2020Article
- Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface.International journal of molecular sciences · 2020Article
- Entrainment of mammalian motile cilia in the brain with hydrodynamic forces.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Biomimetics of the pulmonary environmentBiomicrofluidics · 2018Article
- Long-term preservation of planar cell polarity in reversed tracheal epithelium.Respiratory research · 2018Article
- Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organization of airway epithelium determines ciliary beat direction and coordination for proper mucociliary clearance. Fluidic shear stresses have the potential to influence ciliary organization. Here, an in vitro fluidic flow system was developed for inducing long-term airflow shear stresses on airway epithelium with a view to influencing epithelial organization. Our system consists of a fluidic device for cell culture, integrated into a humidified airflow circuit. The fluidic device has a modular design and is made from a combination of polystyrene and adhesive components incorporated into a 6-well filter membrane insert. We demonstrate the system operates within physiologically relevant shear and pressure ranges and estimate the shear stress exerted on the epithelial cell layer as a result of air flow using a computational model. For both the bronchial epithelial cell line BEAS2B and primary human tracheal airway epithelial cells, we demonstrate that cells remain viable within the device when exposed to airflow for 24 h and that normal differentiation and cilia formation occurs. Furthermore, we demonstrate the utility of our device for exploring the impact of exposing cells to airflow: our tool enables quantification of cytoskeletal organization, and is compatible with in situ bead assays to assess the orientation of cilia beating.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.