ArticleInternational journal of biological sciences2015
Ligands binding and molecular simulation: the potential investigation of a biosensor based on an insect odorant binding protein.
Article in International journal of biological sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Field Application of Tea Volatiles Mediating the Selectivity ofPlants (Basel, Switzerland) · 2025Article
- Evaluating the insecticidal potential of alkaloids for the management of Thrips palmi: in vivo and in silico perspectives.Scientific reports · 2024Article
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- Current Trends and Changes in Use of Membrane Molecular Dynamics Simulations within Academia and the Pharmaceutical Industry.Membranes · 2023Review
- Artificial intelligence in molecular imaging.Annals of translational medicine · 2021Review
- Identification and motif analyses of candidate nonreceptor olfactory genes of Dendroctonus adjunctus Blandford (Coleoptera: Curculionidae) from the head transcriptome.Scientific reports · 2020Article
- Effects of Hydroxyl Group on the Interaction of Carboxylated Flavonoid Derivatives with S. Cerevisiae α-Glucosidase.Current computer-aided drug design · 2020Article
- Biosensors to Monitor Water Quality Utilizing Insect Odorant-Binding Proteins as Detector Elements.Biosensors · 2019Article
- Key site residues of pheromone-binding protein 1 involved in interacting with sex pheromone components of Helicoverpa armigera.Scientific reports · 2017Article
- Computer-aided rational design of novel EBF analogues with an aromatic ring.Journal of molecular modeling · 2016Article
- Structure-Based Analysis of the Ligand-Binding Mechanism for DhelOBP21, a C-minus Odorant Binding Protein, from Dastarcus helophoroides (Fairmaire; Coleoptera: Bothrideridae).International journal of biological sciences · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Based on mimicking biological olfaction, biosensors have been applied for the detection of various ligands in complex environment, which could represent one of the most promising research fields. In this study, the basic characters of one insect odorant binding protein (OBP) as a biosensor were explored. To explore the molecular recognition process, the tertiary structure of the protein was modeled and the protein-ligand interactions with 1,536,550 chemicals were investigated by the molecular docking. The availability of large amount of recombinant SlitOBP1 overcame the difficulty to obtain biological sensing material. After obtained the purified recombinant protein, the result of fluorescence binding assays proved the candidate protein has good affinities with the majority of the tested chemicals. With the aid of simulation docking, the key conserved amino acids within the binding site were identified and then mutated to alanine. After mutation, the protein-ligand binding characteristics were recorded, and the competitive binding assays were carried out to provide experimental verification. The detailed information on its structure and affinities investigated in this study could allow the design of specific mutants with desired characteristics, which provides a solid base for tailoring OBP for biosensor and provides a role model for screening the other elements in olfactory system for different applications.
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