ArticleNature protocols2015
Generation of living cell arrays for atomic force microscopy studies.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Zooming into Disease at the Nanoscale: High-Speed Atomic Force Microscopy in Biomedical Discovery.ACS nano · 2026Review
- Surface-dependent reorganization ofCell surface (Amsterdam, Netherlands) · 2025Article
- Displacement Mapping as a Highly Flexible Surface Texturing Tool for Additively Photopolymerized Components.Micromachines · 2024Article
- Single-cell patterning and characterisation of antibiotic persistent bacteria using bio-sCAPA.Lab on a chip · 2023Article
- Yeast cell wall extracts fromFrontiers in immunology · 2023Article
- Development and Use of a Monoclonal Antibody Specific for theFrontiers in cellular and infection microbiology · 2022Article
- Article
- A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis.Microorganisms · 2021Article
- Article
- Patterning of Particles and Live Cells at Single Cell Resolution.Micromachines · 2020Article
- Using Atomic Force Microscopy To Illuminate the Biophysical Properties of Microbes.ACS applied bio materials · 2020Article
- Microbial adhesion and ultrastructure from the single-molecule to the single-cell levels by Atomic Force Microscopy.Cell surface (Amsterdam, Netherlands) · 2019Review
- QCM-D characterization of time-dependence of bacterial adhesion.Cell surface (Amsterdam, Netherlands) · 2019Review
- AFM dendritips functionalized with molecular probes specific to cell wall polysaccharides as a tool to investigate cell surface structure and organization.Cell surface (Amsterdam, Netherlands) · 2019Article
- Effect of trans(NO, OH)-[RuFT(Cl)(OH)NO](PFScientific reports · 2019Article
- A conserved fungal hub protein involved in adhesion and drug resistance in the human pathogenCell surface (Amsterdam, Netherlands) · 2018Article
- Algal Viruses: The (Atomic) Shape of Things to Come.Viruses · 2018Review
- Atomic Force Microscopy: A Promising Tool for Deciphering the Pathogenic Mechanisms of Fungi in Cystic Fibrosis.Mycopathologia · 2018Review
- Methods of Micropatterning and Manipulation of Cells for Biomedical Applications.Micromachines · 2017Review
- β-(1,3)-Glucan Unmasking in Some Candida albicans Mutants Correlates with Increases in Cell Wall Surface Roughness and Decreases in Cell Wall Elasticity.Infection and immunity · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Atomic force microscopy (AFM) is a useful tool for studying the morphology or the nanomechanical and adhesive properties of live microorganisms under physiological conditions. However, to perform AFM imaging, living cells must be immobilized firmly enough to withstand the lateral forces exerted by the scanning tip, but without denaturing them. This protocol describes how to immobilize living cells, ranging from spores of bacteria to yeast cells, into polydimethylsiloxane (PDMS) stamps, with no chemical or physical denaturation. This protocol generates arrays of living cells, allowing statistically relevant measurements to be obtained from AFM measurements, which can increase the relevance of results. The first step of the protocol is to generate a microstructured silicon master, from which many microstructured PDMS stamps can be replicated. Living cells are finally assembled into the microstructures of these PDMS stamps using a convective and capillary assembly. The complete procedure can be performed in 1 week, although the first step is done only once, and thus repeats can be completed within 1 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.