ArticleNature protocols2015
Protein reconstitution into freestanding planar lipid membranes for electrophysiological characterization.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
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- Fabrication of cytotoxic mirror image nanopores.Nature communications · 2025Article
- Aetokthonotoxin, the Causative Agent of Vacuolar Myelinopathy, Uncouples Oxidative Phosphorylation due to Protonophore Activity.Chemical research in toxicology · 2025Article
- Hetero-Oligomeric Protein Pores for Single-Molecule Sensing.The Journal of membrane biology · 2025Review
- Giant unilamellar vesicles as a model system for studying ion transport.Biophysical reviews · 2025Review
- Gamma-Aminobutyric Acid Action on Membrane and Electrical Properties of Synaptosomes and Model Lipid Bilayers.The Journal of membrane biology · 2025Article
- How to isolate channel-forming membrane proteins using the E. coli expression system.Nature protocols · 2025Review
- "Meet the IUPAB councilor"-Thomas Gutsmann.Biophysical reviews · 2024Article
- Conformational flexibility driving charge-selective substrate translocation across a bacterial transporter.Chemical science · 2024Article
- Naphthalene Diimide-Based Metallacage as an Artificial Ion Channel for Chloride Ion Transport.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Interaction of KLAKLAK-NHPharmaceutics · 2024Article
- Computational drug development for membrane protein targets.Nature biotechnology · 2024Review
- Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans.The Journal of biological chemistry · 2024Article
- Revealing the single-channel characteristics of OprD (OccAB1) porins from hospital strains of Acinetobacter baumannii.European biophysics journal : EBJ · 2023Article
- Erythrocyte Membrane Biophysical Changes Mediated by Pooled Immunoglobulin G and Hematin: Electrokinetic and Lipid Peroxidation Studies.Membranes · 2023Article
- Solid and Liquid Surface-Supported Bacterial Membrane Mimetics as a Platform for the Functional and Structural Studies of Antimicrobials.Membranes · 2022Review
- Assembly of transmembrane pores from mirror-image peptides.Nature communications · 2022Article
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5 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reconstitution of channel-forming proteins into planar lipid bilayers enables their functional characterization at very low (sometimes below attomolar) concentrations. We describe the three main approaches used in our laboratories (the Mueller-Rudin technique, in which the bilayers contain an organic solvent, the Montal-Mueller or solvent-free technique, and a method for membrane reconstitution via liposome formation), and we discuss their respective advantages and limitations. Despite the differences in the reconstitution procedures, subsequent protein characterization is based on the same electrophysiological technique. A transmembrane electric field is applied, inducing an ion current and allowing conclusions to be drawn on apparent pore sizes, or suggesting functional properties such as channel opening and closing upon ligand binding, pH-induced conformational changes, ion selectivity or substrate specificity.
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