ArticlePloS one2014
New insight into secreted ribonuclease structure: binase is a natural dimer.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Structural and Functional Differences between Homologous Bacterial Ribonucleases.International journal of molecular sciences · 2022Article
- RNase A Domain-Swapped Dimers Produced Through Different Methods: Structure-Catalytic Properties and Antitumor Activity.Life (Basel, Switzerland) · 2021Article
- The Cytotoxicity of RNase-Derived Peptides.Biomolecules · 2020Article
- Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.Frontiers in immunology · 2019Review
- The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly.BioMed research international · 2018Article
- Statistical prediction of protein structural, localization and functional properties by the analysis of its fragment mass distributions after proteolytic cleavage.Scientific reports · 2016Article
- The Role of Metals in the Reaction Catalyzed by Metal-Ion-Independent Bacillary RNase.Bioinorganic chemistry and applications · 2016Article
- Three-step procedure for preparation of pure Bacillus altitudinis ribonuclease.FEBS open bio · 2016Article
- Extracellular Ribonuclease fromBioMed research internationalArticle
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biological effects of ribonucleases (RNases), such as the control of the blood vessels growth, the toxicity towards tumour cells and antiviral activity, require a detailed explanation. One of the most intriguing properties of RNases which can contribute to their biological effects is the ability to form dimers, which facilitates efficient RNA hydrolysis and the evasion of ribonuclease inhibitor. Dimeric forms of microbial RNase binase secreted by Bacillus pumilus (former B. intermedius) have only been found in crystals to date. Our study is the first report directly confirming the existence of binase dimers in solution and under natural conditions of enzyme biosynthesis and secretion by bacilli. Using different variants of gel electrophoresis, immunoblotting, size-exclusion chromatography and mass-spectrometry, we revealed that binase is a stable natural dimer with high catalytic activity.
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Registered trials
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