ArticleInternational journal of clinical and experimental pathology2014
α-Naphthoflavone modulates inflammatory response in adipocytes-macrophages interaction through NFκB signaling.
Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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9 authors at 1 institution in 1 country.
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Abstract
objectiveOur previous study demonstrated that α-naphthoflavone (α-NF) inhibits mouse 3T3-L1 pre-adipocytes differentiation via PPARγ, a key transcription factor in adipogenesis. Due to the critical role of inflammation in adipogenesis, we speculated that the suppression role of α-NF in adipogenesis might involve in modulation of cytokines secretion raised by adipocyte differentiation cocktail. Therefore, the present study aims to investigate the role of α-NF in modulating of inflammatory response during adipocytes differentiation and adipocyte-macrophage interaction.
methodsConditioned medium from different doses of α-NF treated 10-day differentiated 3T3-L1 adipocytes were collected to culture RAW264.7 macrophages. Conditioned medium from activated macrophages and α-NF pre-treated macrophage were used to investigate the effects of α-NF in adipocytes differentiation. Cultured cells and medium were harvested for RT-PCR, Western blot and ELISA.
resultsα-NF dose-dependently decreased TNF-α and IL-6 and increased IL-10 expression induced by IDM (Insulin, dexamethasone, isobutylmethylxanthine) in 3T3-L1 pre-adipocytes. Conditioned medium from α-NF treated 3T3-L1 differentiated cells inhibited inflammatory response in mouse macrophage cell line RAW264.7 in contrast to IDM control medium. NFĸB activation elicited by IDM was suppressed by α-NF in a dose-response manner. Consequently, decreased TNF-α and increased IL-10 secretion, downstream targets of NFĸB signaling pathway, were observed with α-NF in macrophages. Finally, Conditioned medium from α-NF pre-treated, LPS-activated macrophages ameliorated the suppression of 3T3-L1 adipogenesis by LPS activated macrophages.
conclusionOur results suggest that α-NF regulates inflammation response in both adipocytes and macrophages and adipocyte-macrophage interaction which contributes to pre-adipocyte differentiation.
Indexed as
Identifiers
25550814PMC4270567W2200390719What OpenQuestion holds
Registered trials
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