ArticleInternational journal of clinical and experimental pathology2014
The role of integrin-β/FAK in cyclic mechanical stimulation in MG-63 cells.
Article in International journal of clinical and experimental pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Biomechanical Aspects in Bone Tumor Engineering.Tissue engineering. Part B, Reviews · 2024Review
- Applications of Bioinspired Platforms for Enhancing Immunomodulatory Function of Mesenchymal Stromal Cells.International journal of stem cells · 2023Review
- An overview of resistance to chemotherapy in osteosarcoma and future perspectives.Cancer drug resistance (Alhambra, Calif.) · 2022Review
- Evidence of Modular Responsiveness of Osteoblast-Like Cells Exposed to Hydroxyapatite-Containing Magnetic Nanostructures.Biology · 2020Article
- Targeting Mechanotransduction in Osteosarcoma: A Comparative Oncology Perspective.International journal of molecular sciences · 2020Review
- Review
- Orthodontic compressive force modulates Ets-1/Tks5 pathway to promote the formation of circumferential invadopodia and the fusion of osteoclast precursors.Journal of cellular physiology · 2019Article
- Regulation of Cell Behavior by Hydrostatic Pressure.Applied mechanics reviews · 2019Review
- Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.Biomaterials · 2019Article
- Cyclic Tensile Strain Upregulates Pro-Inflammatory Cytokine Expression Via FAK-MAPK Signaling in Chondrocytes.Inflammation · 2018Article
- Osteoblast-targeted delivery of miR-33-5p attenuates osteopenia development induced by mechanical unloading in mice.Cell death & disease · 2018Article
- 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response.BMC cancer · 2016Article
- miR-33-5p, a novel mechano-sensitive microRNA promotes osteoblast differentiation by targeting Hmga2.Scientific reports · 2016Article
- IGF-binding protein 2 is a candidate target of therapeutic potential in cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study aims to explore the function of Integrin-β/FAK in the mechanical signal transduction and the connection with downstream ERK signal pathways.
methodsHuman osteosarcoma MG63 cell lines were used in this study. The effects of mechanical strain on the Integrin-β₁ expression, FAK and ERK signal pathway in Human osteosarcoma MG63 cells were detected using RT-PCR and Western-blotting methods. The localization of FAK in Human osteosarcoma MG63 cells were determined using immunofluorescent method. The interaction between Integrin-β₁ and FAK were detected by using co-immunoprecipitation method.
resultsThe expression of Integrin-β₁ shows a notable bimodel distribution, mechanical strain stimulation can promote Integrin-β₁ expression and the phosphorylation of FAK and ERK, mechanical strain activated FAK and ERK mediated by Integrin-β₁.
conclusionIntegrin-β₁ may play an important role in osteoblast proliferation differentiation process, it might feel external strain stimulation through ECM composition and makes FAK phosphated through the interaction with FAK, thus causing a series of activation of signal molecules. Finally it reduces MAPK (ERK) activation and cellular responses to finish mechanical signal transduction.
Indexed as
Identifiers
25550780PMC4270589W1691622795What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.