ArticleProceedings of the National Academy of Sciences of the United States of America2015
Nitric oxide negatively regulates abscisic acid signaling in guard cells by S-nitrosylation of OST1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.
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Who cites it
136 citing papers in PubMed, 353 citations in OpenAlex.
- Abscisic acid interplay with phytohormones, plant growth regulators and signaling molecules in orchestrating an elaborate crosstalk for stress resilience in plants.Biologia futura · 2026Review
- Nitric oxide interacts with brassinosteroid signalling to regulate plant growth and development in an organ-specific manner.Journal of experimental botany · 2026Article
- HHorticulture research · 2026Article
- Excess nitric oxide alters cellular pH to restrict salicylic acid movement and systemic immunity.Science advances · 2026Article
- From odor to order: unveiling the crucial role of hydrogen sulfide in plant life.Horticulture research · 2026Article
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- The Central Role of GSNOR: Decoding Nitric Oxide Signaling for Crop Stress Tolerance.International journal of molecular sciences · 2025Review
- Article
- Nitric Oxide and Photosynthesis Interplay in Plant Interactions with Pathogens.International journal of molecular sciences · 2025Review
- Nitric Oxide Regulates Multiple Signal Pathways in Plants via ProteinCurrent issues in molecular biology · 2025Review
- SnRK2s: Kinases or Substrates?Plants (Basel, Switzerland) · 2025Review
- SnRK2 kinases sense molecular crowding and form condensates to disrupt ABI1 inhibition.Science advances · 2025Article
- Dressed Up to the Nines: The Interplay of Phytohormones Signaling and Redox Metabolism During Plant Response to Drought.Plants (Basel, Switzerland) · 2025Review
- Nitric oxide regulates photosynthesis and the capacity of the antioxidant system under water deficit and rehydration inFrontiers in plant science · 2025Article
- Assessing the role of exogenous NO on plants and microbial communities in soil.ISME communications · 2025Article
- Nitric oxide in plants: an insight on redox activity and responses toward abiotic stress signaling.Plant signaling & behavior · 2024Review
- Starch metabolism in guard cells: At the intersection of environmental stimuli and stomatal movement.Plant physiology · 2024Review
- Post-Translational Modifications to Cysteine Residues in Plant Proteins and Their Impact on the Regulation of Metabolism and Signal Transduction.International journal of molecular sciences · 2024Review
- Role of protein S-nitrosylation in plant growth and development.Plant cell reports · 2024Review
- Nitric Oxide Mitigates the Deleterious Effects Caused by Infection ofPlants (Basel, Switzerland) · 2024Article
76 more citing papers are in PubMed but not listed here.
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10 authors at 4 institutions in 2 countries.
Funding
Abstract
The phytohormone abscisic acid (ABA) plays important roles in plant development and adaptation to environmental stress. ABA induces the production of nitric oxide (NO) in guard cells, but how NO regulates ABA signaling is not understood. Here, we show that NO negatively regulates ABA signaling in guard cells by inhibiting open stomata 1 (OST1)/sucrose nonfermenting 1 (SNF1)-related protein kinase 2.6 (SnRK2.6) through S-nitrosylation. We found that SnRK2.6 is S-nitrosylated at cysteine 137, a residue adjacent to the kinase catalytic site. Dysfunction in the S-nitrosoglutathione (GSNO) reductase (GSNOR) gene in the gsnor1-3 mutant causes NO overaccumulation in guard cells, constitutive S-nitrosylation of SnRK2.6, and impairment of ABA-induced stomatal closure. Introduction of the Cys137 to Ser mutated SnRK2.6 into the gsnor1-3/ost1-3 double-mutant partially suppressed the effect of gsnor1-3 on ABA-induced stomatal closure. A cysteine residue corresponding to Cys137 of SnRK2.6 is present in several yeast and human protein kinases and can be S-nitrosylated, suggesting that the S-nitrosylation may be an evolutionarily conserved mechanism for protein kinase regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.