Evidence map›Paper›PMID 25550365›Full record

ArticleStroke2015

Norrin protected blood-brain barrier via frizzled-4/β-catenin pathway after subarachnoid hemorrhage in rats.

Yujie Chen, Yang Zhang, Junjia Tang, Fei Liu, Qin Hu, Chunxia Luo, Jiping Tang, Hua Feng, John H Zhang

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stroke, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
7.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 95 citations in OpenAlex.

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2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Yujie ChenFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Yang ZhangFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Junjia TangFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Fei LiuFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Qin HuFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Chunxia LuoFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Jiping TangFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
Hua FengFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.).
John H ZhangFrom the Departments of Neurosurgery (Y.C., H.F.) and Neurology (C.L.), Southwest Hospital, Third Military Medical University, Chongqing, China; and Department of Physiology and Pharmacology, Loma Linda University, CA (Y.C., Y.Z., J.T., F.L., Q.H., J.T., J.H.Z.). johnzhang3910@yahoo.com fenghua8888@vip.163.com.
Army Medical University · CN

Funding

Vascular Biology Core FacilityP01NS082184 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2014 to 2018
$6.1M
Crotalus Snake Venom Preconditioning to Prevent Surgical Brain InjuryR01NS084921 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2014 to 2018
$1.7M
Neurovascular Protection for Early Brain Injury after SAHR01NS081740 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2013 to 2017
$1.6M
NINDS NIH HHS NS081740NINDS NIH HHS NS084921NINDS NIH HHS P01 NS082184NINDS NIH HHS R01 NS081740NINDS NIH HHS R01 NS084921
6 · The paper itself

Abstract

background and purposeNorrin and its receptor Frizzled-4 have important roles in the blood-brain barrier development. This study is to investigate a potential role and mechanism of Norrin/Frizzled-4 on protecting blood-brain barrier integrity after subarachnoid hemorrhage (SAH).

methodsOne hundred and seventy-eight male Sprague-Dawley rats were used. SAH model was induced by endovascular perforation. Frizzled-4 small interfering RNA was injected intracerebroventricularly 48 hours before SAH. Norrin was administrated intracerebroventricularly 3 hours after SAH. SAH grade, neurological scores, brain water content, Evans blue extravasation, western blots, and immunofluorescence were used to study the mechanisms of Norrin and its receptor regulation protein TSPAN12, as well as neurological outcome.

resultsEndogenous Norrin and TSPAN12 expression were increased after SAH, and Norrin was colocalized with astrocytes marker glial fibrillary acidic protein in cortex. Exogenous Norrin treatment significantly alleviated neurobehavioral dysfunction, reduced brain water content and Evans blue extravasation, promoted β-catenin nuclear translocation, and increased Occludin, VE-Cadherin, and ZO-1 expressions. These effects were abolished by Frizzled-4 small interfering RNA pretreated before SAH.

conclusionsNorrin protected blood-brain barrier integrity and improved neurological outcome after SAH, and the action of Norrin appeared mediated by Frizzled-4 receptor activation, which promoted β-catenin nuclear translocation, which then enhanced Occludin, VE-Cadherin, and ZO-1 expression. Norrin might have potential to protect blood-brain barrier after SAH.

Indexed as

Animalsbeta CateninBlood-Brain BarrierEye ProteinsFrizzled ReceptorsInjections, IntraventricularMaleNerve Tissue ProteinsRandom AllocationRatsRats, Sprague-DawleySubarachnoid Hemorrhagebeta CateninCtnnb1 protein, ratEye ProteinsFrizzled ReceptorsFzd4 protein, ratNDP protein, humanNerve Tissue Proteinsblood–brain barrierFrizzled-4 proteinNorrinsubarachnoid hemorrhage

Identifiers

PMID25550365
PMCPMC4308484
OpenAlexW2125960717

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.