Evidence map›Paper›PMID 25549949›Full record

ArticleReproductive toxicology (Elmsford, N.Y.)2015

Methoxychlor and its metabolite HPTE inhibit cAMP production and expression of estrogen receptors α and β in the rat granulosa cell in vitro.

Craig N Harvey, Joseph C Chen, Carol A Bagnell, Mehmet Uzumcu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive toxicology (Elmsford, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Craig N HarveyDepartment of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA; Joint Graduate Program in Toxicology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
Joseph C ChenDepartment of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
Carol A BagnellDepartment of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
Mehmet UzumcuDepartment of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA; Joint Graduate Program in Toxicology, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA. Electronic address: uzumcu@aesop.rutgers.edu.
Rutgers, The State University of New Jersey · US

Funding

Translational Research Support CoreP30ES005022 · NIEHS · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI ROBERT John LAUMBACH · 1988 to 2026
$47.4M
TRAINING IN EVIRONMENTAL TOXICOLOGYT32ES007148 · NIEHS · RUTGERS THE ST UNIV OF NJ NEW BRUNSWICK · PI Lauren M Aleksunes · 1987 to 2026
$11.4M
Epigenetic transgenerational effects of endocrine disruptors via female germ lineR21ES017847 · NIEHS · RUTGERS, THE STATE UNIV OF N.J. · PI UZUMCU, MEHMET · 2011 to 2013
$619k
EPIGENETIC EFFECT OF METHOXYCHLOR ON OVARIAN DEVELOPMENTR21ES013854 · NIEHS · RUTGERS, THE STATE UNIV OF N.J. · PI UZUMCU, MEHMET · 2006 to 2007
$391k
Epigenetic effects of developmental endocrine disruptor exposure in the ovaryR56ES017059 · NIEHS · RUTGERS, THE STATE UNIV OF N.J. · PI UZUMCU, MEHMET · 2010 to 2010
$154k
NIEHS NIH HHS ES005022NIEHS NIH HHS ES013854NIEHS NIH HHS ES017059NIEHS NIH HHS ES017847NIEHS NIH HHS P30 ES005022NIEHS NIH HHS R21 ES013854NIEHS NIH HHS R21 ES017847NIEHS NIH HHS R56 ES017059NIEHS NIH HHS T32 ES007148
6 · The paper itself

Abstract

The major metabolite of the estrogenic pesticide methoxychlor (MXC) HPTE is a stronger ESR1 agonist than MXC and acts also as an ESR2 antagonist. In granulosa cells (GCs), FSH stimulates estradiol via the second messenger cAMP. HPTE inhibits estradiol biosynthesis, and this effect is greater in FSH-treated GCs than in cAMP-treated GCs. Therefore; we examined the effect of MXC/HPTE on FSH-stimulated cAMP production in cultured GCs. To test involvement of ESR-signaling, we used the ESR1 and ESR2 antagonist ICI 182,780, ESR2 selective antagonist PHTPP, and ESR2 selective agonist DPN. ESR1 and ESR2 mRNA and protein levels were quantified. Both HPTE and MXC inhibited the FSH-induced cAMP production. ICI 182,780 and PHTPP mimicked the inhibitory action of HPTE. MXC/HPTE reduced FSH-stimulated Esr2 mRNA and protein to basal levels. MXC/HPTE also inhibited FSH-stimulated Esr1. The greater inhibition on FSH-stimulated GCs is likely due to reduced cAMP level that involves ESR-signaling, through ESR2.

Indexed as

AnimalsCells, CulturedCyclic AMPEstradiolEstrogen Receptor alphaEstrogen Receptor betaEstrogen Receptor ModulatorsFemaleFollicle Stimulating HormoneFulvestrantGranulosa CellsInsecticidesMethoxychlorNitrilesPhenolsPropionates2,2-bis(4-hydroxyphenyl)-1,1,1-trichloroethane2,3-bis(4-hydroxyphenyl)-propionitrile4-(2-phenyl-5,7-bis(trifluoromethyl)pyrazolo(1,5-a)pyrimidin-3-yl)phenolCyclic AMPEstradiolEstrogen Receptor alphaEstrogen Receptor betaEstrogen Receptor ModulatorsFollicle Stimulating HormoneFulvestrantInsecticidesMethoxychlorNitrilesPhenolsPropionatesPyrazolesPyrimidinesRNA, MessengerCyclic AMPEndocrine disruptorsEstrogen receptorsFollicle-stimulating hormoneHPTEMethoxychlorOvary

Identifiers

PMID25549949
PMCPMC4465425
OpenAlexW2050670539

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.