ArticlePloS one2014
Rapid and sensitive detection of Listeria ivanovii by loop-mediated isothermal amplification of the smcL gene.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Recent Development in Detection and Control of Psychrotrophic Bacteria in Dairy Production: Ensuring Milk Quality.Foods (Basel, Switzerland) · 2024Review
- Aptamer-associated colorimetric reverse transcription loop-mediated isothermal amplification assay for detection of dengue virus.Microbiology spectrum · 2024Article
- Virulence Characteristics and Distribution of the Pathogen Listeria ivanovii in the Environment and in FoodMicroorganisms · 2022Review
- Detection of Mycobacterium kansasii using a combination of loop-mediated isothermal amplification (LAMP) and lateral flow biosensors.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Article
- Rapid Detection of Hypervirulent Serovar 4hFrontiers in microbiology · 2020Article
- Development and Application of an Immunocapture PCR Diagnostic Assay Based on the Monoclonal Antibody for the Detection ofIranian journal of biotechnology · 2020Article
- Detection ofFrontiers in microbiology · 2018Article
- Rapid, Sensitive Detection of Bartonella quintana by Loop-Mediated Isothermal Amplification of the groEL Gene.International journal of molecular sciences · 2016Article
- Rapid and Sensitive Detection of Vibrio parahaemolyticus and Vibrio vulnificus by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique.Molecules (Basel, Switzerland) · 2016Article
- The Novel Multiple Inner Primers-Loop-Mediated Isothermal Amplification (MIP-LAMP) for Rapid Detection and Differentiation of Listeria monocytogenes.Molecules (Basel, Switzerland) · 2015Article
- Rapid and Sensitive Detection of Shigella spp. and Salmonella spp. by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique.Frontiers in microbiology · 2015Article
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Authors and funding
6 authors.
Funding
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Abstract
A loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive detection of the L. ivanovii strains had been developed and evaluated in this study. Oligonucleotide primers specific for L. ivanovii species were designed corresponding to smcL gene sequences. The primers set comprise six primers targeting eight regions on the species-specific gene smcL. The LAMP assay could be completed within 1 h at 64°C in a water bath. Amplification products were directly observed by the Loopamp Fluorescent Detection Reagent (FD) or detected by agarose gel electrophoresis. Moreover, the LAMP reactions were also detected by real-time measurement of turbidity. The exclusivity of 77 non-L. ivanovii and the inclusivity of 17 L. ivanovii were both 100% in the assay. Sensitivity of the LAMP assay was 250 fg DNA and 16 CFU per reaction for detection of L. ivanovii in pure cultures and simulated human stool. The LAMP assay was 10 and 100-fold more sensitive than quantitative PCR (qPCR) and conventional PCR assays,respectively. When applied to human stool samples spiked with low level (8 CFU/0.5 g) of L. ivanovii strains, the new LAMP assay described here achieved positive detection after 6 hours enrichment. In conclusion, the new LAMP assay in this study can be used as a valuable, rapid and sensitive detection tool for the detection of L. ivanovii in field, medical and veterinary laboratories.
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