ArticleeLife2014
Specificity in endoplasmic reticulum-stress signaling in yeast entails a step-wise engagement of HAC1 mRNA to clusters of the stress sensor Ire1.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Functional Characterization ofInternational journal of molecular sciences · 2026Article
- The transcription factor CfHac1 regulates the degradation of ubiquitin-mediated ER-associated misfolded proteins and pathogenicity in Colletotrichum fructicola.Stress biology · 2025Article
- Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering.The EMBO journal · 2024Article
- Congress of multiple dimers is needed for cross-phosphorylation of IRE1α and its RNase activity.Life science alliance · 2024Article
- Mammalian IRE1α dynamically and functionally coalesces with stress granules.Nature cell biology · 2024Article
- In Silico Analysis of Protein-Protein Interactions of Putative Endoplasmic Reticulum Metallopeptidase 1 inCurrent issues in molecular biology · 2024Article
- Fundamental and Applicative Aspects of the Unfolded Protein Response in Yeasts.Journal of fungi (Basel, Switzerland) · 2023Review
- Andrographolide Induces ROS-Mediated Cytotoxicity, Lipid Peroxidation, and Compromised Cell Integrity inAntioxidants (Basel, Switzerland) · 2023Article
- Termination of the unfolded protein response is guided by ER stress-induced HAC1 mRNA nuclear retention.Nature communications · 2022Article
- Article
- Determination of the Stability and Intracellular (Intra-Nuclear) Targeting and Recruitment of Pre-HAC1 mRNA in the Saccharomyces cerevisiae During the Activation of UPR.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Cysteine cross-linking in native membranes establishes the transmembrane architecture of Ire1.The Journal of cell biology · 2021Article
- Multiple Ways for Stress Sensing and Regulation of the Endoplasmic Reticulum-stress Sensors.Cell structure and function · 2021Review
- DoesMicroorganisms · 2021Article
- Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress.The Journal of cell biology · 2020Article
- Quantitative microscopy reveals dynamics and fate of clustered IRE1α.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Transcriptomic Analysis ofFrontiers in microbiology · 2020Article
- Clustering of IRE1α depends on sensing ER stress but not on its RNase activity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Translation Control ofInternational journal of molecular sciences · 2019Review
- Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Insufficient protein-folding capacity in the endoplasmic reticulum (ER) induces the unfolded protein response (UPR). In the ER lumen, accumulation of unfolded proteins activates the transmembrane ER-stress sensor Ire1 and drives its oligomerization. In the cytosol, Ire1 recruits HAC1 mRNA, mediating its non-conventional splicing. The spliced mRNA is translated into Hac1, the key transcription activator of UPR target genes that mitigate ER-stress. In this study, we report that oligomeric assembly of the ER-lumenal domain is sufficient to drive Ire1 clustering. Clustering facilitates Ire1's cytosolic oligomeric assembly and HAC1 mRNA docking onto a positively charged motif in Ire1's cytosolic linker domain that tethers the kinase/RNase to the transmembrane domain. By the use of a synthetic bypass, we demonstrate that mRNA docking per se is a pre-requisite for initiating Ire1's RNase activity and, hence, splicing. We posit that such step-wise engagement between Ire1 and its mRNA substrate contributes to selectivity and efficiency in UPR signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.