ArticleThe Biochemical journal1989
5'-Nucleotidases of chicken gizzard and human pancreatic adenocarcinoma cells are anchored to the plasma membrane via a phosphatidylinositol-glycan.
Article in The Biochemical journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- RNA interference of ecto-5'-nucleotidase (CD73) inhibits human breast cancer cell growth and invasion.Clinical & experimental metastasis · 2007Article
- The effect of muscle contraction on the regulation of adenosine formation in rat skeletal muscle cells.The Journal of physiology · 1999Article
- Release of the glycosylphosphatidylinositol-anchored enzyme ecto-5'-nucleotidase by phospholipase C: catalytic activation and modulation by the lipid bilayer.The Biochemical journal · 1998Article
- Reconstitution of 5'-nucleotidase of bull seminal plasma in spin-labeled liposomes.The Journal of membrane biology · 1994Article
- Isolation and characterization of the ecto-5'-nucleotidase from a rat glioblastoma cell line.Molecular and cellular biochemistry · 1992Article
- 5'-Nucleotidase: molecular structure and functional aspects.The Biochemical journal · 1992Review
- Soluble low-Km 5'-nucleotidase from electric-ray (Torpedo marmorata) electric organ and bovine cerebral cortex is derived from the glycosyl-phosphatidylinositol-anchored ectoenzyme by phospholipase C cleavage.The Biochemical journal · 1992Article
- Membrane sorting during phagocytosis: selective exclusion of major histocompatibility complex molecules but not complement receptor CR3 during conventional and coiling phagocytosis.The Journal of experimental medicine · 1992Article
- Modulation of 5'-nucleotidase activity in plasma membranes and intact cells by the extracellular matrix proteins laminin and fibronectin.The Biochemical journal · 1992Article
- Factors affecting the ability of glycosylphosphatidylinositol-specific phospholipase D to degrade the membrane anchors of cell surface proteins.The Biochemical journal · 1991Article
- Association of the HNK-1 epitope with 5'-nucleotidase from Torpedo marmorata (electric ray) electric organ.The Biochemical journal · 1991Article
- Actin binding proteins--lipid interactions.Journal of muscle research and cell motility · 1991Review
- Emerging functional roles for the glycosyl-phosphatidylinositol membrane protein anchor.The Journal of membrane biology · 1990Review
- Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart.The Biochemical journal · 1990Article
- Affinity labelling of 5'-nucleotidases with 5'-p-fluorosulphonylbenzoyladenosine.The Biochemical journal · 1990Article
- Inositol is a constituent of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase.The Biochemical journal · 1990Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have analysed the membrane anchorage of plasma-membrane 5'-nucleotidase, an ectoenzyme which can mediate binding to components of the extracellular matrix. We demonstrated that the purified enzyme obtained from chicken gizzard and a human pancreatic adenocarcinoma cell line were both completely transformed into a hydrophilic form by treatment with phospholipases C and D, cleaving glycosylphosphatidylinositol (GPI). These data indicate the presence of a glycolipid linker employed for membrane anchoring of the 5'-nucleotidase obtained from both sources. Incubation of plasma membranes under identical conditions revealed that about half of the AMPase activity was resistant to GPI-hydrolysing phospholipases. Investigation of the enzymic properties of purified chicken gizzard 5'-nucleotidase revealed only minor changes after removal of the phosphatidylinositol linker. However, cleavage of the membrane anchor resulted in an increased sensitivity towards inhibition by concanavalin A. After tissue fractionation, chicken gizzard 5'-nucleotidase could be obtained as either a membrane-bound or a soluble protein; the latter is suspected to be released from the plasma membrane by endogenous phospholipases. Higher-molecular-mass proteins immuno-cross-reactive with the purified chicken gizzard 5'-nucleotidase were detected as both soluble and membrane-bound forms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.