ArticleBioMed research international2014
Barcoding melting curve analysis for rapid, sensitive, and discriminating authentication of saffron (Crocus sativus L.) from its adulterants.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 55 citations in OpenAlex.
- DART-Triple Quadrupole Mass Spectrometry Method for Multi-Target and Fast Detection of Adulterants in Saffron.Metabolites · 2025Article
- Integration of a high-resolution melt curve assay into a commercial quantification kit for preliminary identification of biological mixtures.International journal of legal medicine · 2025Article
- Spice and Herb Frauds: Types, Incidence, and Detection: The State of the Art.Foods (Basel, Switzerland) · 2023Review
- DNA barcoding in herbal medicine: Retrospective and prospective.Journal of pharmaceutical analysis · 2023Review
- Testing Together Behaviors in Secondary Distribution of HIV/Syphilis Self-testing Program Among Men Who have Sex with Men in China.AIDS and behavior · 2023Article
- Mechanisms of gene regulation by histone degradation in adaptation of yeast: an overview of recent advances.Archives of microbiology · 2022Review
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- DNA Accounting: Tallying Genomes to Detect Adulterated Saffron.Foods (Basel, Switzerland) · 2021Article
- Molecular authentication ofSaudi journal of biological sciences · 2019Article
- Candidate DNA Barcode Tags Combined With High Resolution Melting (Bar-HRM) Curve Analysis for Authentication ofFrontiers in plant science · 2018Article
- Forensic genetics and genomics: Much more than just a human affair.PLoS genetics · 2017Review
- Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR.Scientific reports · 2017Article
- Rapid authentication of the precious herb saffron by loop-mediated isothermal amplification (LAMP) based on internal transcribed spacer 2 (ITS2) sequence.Scientific reports · 2016Article
- Genetic and Epigenetic Approaches for the Possible Detection of Adulteration and Auto-Adulteration in Saffron (Crocus sativus L.) Spice.Molecules (Basel, Switzerland) · 2016Article
- DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market.Plant biotechnology journal · 2016Review
- Authentication of medicinal plants by DNA markers.Plant gene · 2015Review
- DNA-based identification of Calendula officinalis (Asteraceae).Applications in plant sciences · 2015Article
- Identification ofIranian journal of biotechnology · 2015Article
- Saffron'omics': The challenges of integrating omic technologies.Avicenna journal of phytomedicineReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saffron (Crocus sativus L.) is one of the most important and expensive medicinal spice products in the world. Because of its high market value and premium price, saffron is often adulterated through the incorporation of other materials, such as Carthamus tinctorius L. and Calendula officinalis L. flowers, Hemerocallis L. petals, Daucus carota L. fleshy root, Curcuma longa L. rhizomes, Zea may L., and Nelumbo nucifera Gaertn. stigmas. To develop a straightforward, nonsequencing method for rapid, sensitive, and discriminating detection of these adulterants in traded saffron, we report here the application of a barcoding melting curve analysis method (Bar-MCA) that uses the universal chloroplast plant DNA barcoding region trnH-psbA to identify adulterants. When amplified at DNA concentrations and annealing temperatures optimized for the curve analysis, peaks were formed at specific locations for saffron (81.92°C) and the adulterants: D. carota (81.60°C), C. tinctorius (80.10°C), C. officinalis (79.92°C), Dendranthema morifolium (Ramat.) Tzvel. (79.62°C), N. nucifera (80.58°C), Hemerocallis fulva (L.) L. (84.78°C), and Z. mays (84.33°C). The constructed melting curves for saffron and its adulterants have significantly different peak locations or shapes. In conclusion, Bar-MCA could be a faster and more cost-effective method to authenticate saffron and detect its adulterants.
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