ArticleMediators of inflammation2014
Optimized "in vitro" culture conditions for human rheumatoid arthritis synovial fibroblasts.
Article in Mediators of inflammation, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Fasciola hepatica extract suppresses fibroblast-like synoviocytes in vitro and alleviates experimental arthritis.Advances in rheumatology (London, England) · 2022Trial
- Small extracellular vesicles derived from synovial fibroblasts contain distinct miRNA profiles and contribute to chondrocyte damage in osteoarthritis.Arthritis research & therapy · 2024Article
- Connexin 43 Modulation in Human Chondrocytes, Osteoblasts and Cartilage Explants: Implications for Inflammatory Joint Disorders.International journal of molecular sciences · 2024Article
- PRP as a modulator of inflammation in FLS of RA patients by regulation of galectins and TGF-β1.Heliyon · 2024Article
- Pro Nerve Growth Factor and Its Receptor p75NTR Activate Inflammatory Responses in Synovial Fibroblasts: A Novel Targetable Mechanism in Arthritis.Frontiers in immunology · 2022Article
- S100A8 alarmin supports IL-6 and metalloproteinase-9 production by fibroblasts in the synovial microenvironment of peripheral spondyloarthritis.Frontiers in immunology · 2022Article
- Local transplantation of adipose-derived stem cells has a significant therapeutic effect in a mouse model of rheumatoid arthritis.Scientific reports · 2020Article
- Initial cell plating density affects properties of human primary synovial mesenchymal stem cells.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- Th17 and Th1 Lymphocytes in Oligoarticular Juvenile Idiopathic Arthritis.Frontiers in immunology · 2019Review
- Anti-inflammatory Effect of Somatostatin Analogue Octreotide on Rheumatoid Arthritis Synoviocytes.Inflammation · 2018Article
- A promising novel formulation for articular cartilage regeneration: Preclinical evaluation of a treatment that produces SOX9 overexpression in human synovial fluid cells.Molecular medicine reports · 2018Article
- Osthole improves collagen-induced arthritis in a rat model through inhibiting inflammation and cellular stress.Cellular & molecular biology letters · 2018Article
- TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential.PloS one · 2017Article
- Th1-Induced CD106 Expression Mediates Leukocytes Adhesion on Synovial Fibroblasts from Juvenile Idiopathic Arthritis Patients.PloS one · 2016Article
- Smac127 Has Proapoptotic and Anti-Inflammatory Effects on Rheumatoid Arthritis Fibroblast-Like Synoviocytes.Mediators of inflammation · 2016Article
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9 authors.
Funding
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Abstract
The composition of synovial fluid in rheumatoid arthritis (RA) is complex and strongly influences the microenvironment of joints and it is an inseparable element of the disease. Currently, "in vitro" studies are performed on RA cells cultured in the presence of either recombinant proinflammatory cytokines-conditioned medium or medium alone. In this study, we evaluated the use of synovial fluid, derived from RA patients, as optimal culture condition to perform "in vitro" studies on RA synovial fibroblasts. We observed that synovial fluid is more effective in inducing cell proliferation with respect to TNF-alpha or culture medium alone. Spontaneous apoptosis in fibroblasts was also decreased in response to synovial fluid. The expression of proinflammatory cytokines in the presence of synovial fluid was significantly elevated with respect to cells cultured with TNF-alpha or medium, and the overall morphology of cells was also modified. In addition, modulation of intracellular calcium dynamics elicited in response to synovial fluid or TNF-alpha exposure is different and suggests a role for the purinergic signalling in the modulation of the effects. These results emphasize the importance of using RA synovial fluid in "in vitro" studies involving RA cells, in order to reproduce faithfully the physiopathological environmental characteristic of RA joints.
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