ArticleJournal of bacteriology2015
Analysis of metabolism in dormant spores of Bacillus species by 31P nuclear magnetic resonance analysis of low-molecular-weight compounds.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 64 citations in OpenAlex.
- Evolutionary bioenergetics of sporulation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Early activation of bioenergetic metabolism powers bacterial spore germination.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Germination and Heat Resistance ofFoods (Basel, Switzerland) · 2025Article
- OptimizingmSphere · 2025Article
- Investigating the Antibacterial Effect of a Novel Gallic Acid-Based Green Sanitizer Formulation.Foods (Basel, Switzerland) · 2024Article
- DipR, a GntR/FadR-family transcriptional repressor: regulatory mechanism and widespread distribution of the dip cluster for dipicolinic acid catabolism in bacteria.Nucleic acids research · 2024Article
- Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization ofJournal of proteome research · 2024Article
- The role of metabolism in cellular quiescence.Journal of cell science · 2023Review
- New Thoughts on an Old Topic: Secrets of Bacterial Spore Resistance Slowly Being Revealed.Microbiology and molecular biology reviews : MMBR · 2023Review
- Human-Gut Phages Harbor Sporulation Genes.mBio · 2023Article
- Expression of the 2Duf protein in wild-type Bacillus subtilis spores stabilizes inner membrane proteins and increases spore resistance to wet heat and hydrogen peroxide.Journal of applied microbiology · 2023Article
- Role ofMicroorganisms · 2023Article
- Effects of Desiccation and Freezing on Microbial Ionizing Radiation Survivability: Considerations for Mars Sample Return.Astrobiology · 2022Article
- Electrochemical potential enables dormant spores to integrate environmental signals.Science (New York, N.Y.) · 2022Article
- The Novel Monooxygenase GeneApplied and environmental microbiology · 2022Article
- Reviving the view: evidence that macromolecule synthesis fuels bacterial spore germination.microLife · 2022Review
- High Resolution Analysis of Proteome Dynamics duringInternational journal of molecular sciences · 2021Article
- Molecular Physiological Characterization of a High Heat Resistant Spore FormingMicroorganisms · 2021Article
- Bioluminescence dynamics in single germinating bacterial spores reveal metabolic heterogeneity.Journal of the Royal Society, Interface · 2020Article
- Visualization of Germination Proteins in PutativeInternational journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work was undertaken to obtain information on levels of metabolism in dormant spores of Bacillus species incubated for weeks at physiological temperatures. Spores of Bacillus megaterium and Bacillus subtilis strains were harvested shortly after release from sporangia and incubated under various conditions, and dormant spore metabolism was monitored by (31)P nuclear magnetic resonance (NMR) analysis of molecules including 3-phosphoglyceric acid (3PGA) and ribonucleotides. Incubation for up to 30 days at 4, 37, or 50°C in water, at 37 or 50°C in buffer to raise the spore core pH from ∼6.3 to 7.8, or at 4°C in spent sporulation medium caused no significant changes in ribonucleotide or 3PGA levels. Stage I germinated spores of Bacillus megaterium that had slightly increased core water content and a core pH of 7.8 also did not degrade 3PGA and accumulated no ribonucleotides, including ATP, during incubation for 8 days at 37°C in buffered saline. In contrast, spores incubated for up to 30 days at 37 or 50°C in spent sporulation medium degraded significant amounts of 3PGA and accumulated ribonucleotides, indicative of RNA degradation, and these processes were increased in B. megaterium spores with a core pH of ∼7.8. However, no ATP was accumulated in these spores. These data indicate that spores of Bacillus species stored in water or buffer at low or high temperatures exhibited minimal, if any, metabolism of endogenous compounds, even when the spore core pH was 7.8 and core water content was increased somewhat. However, there was some metabolism in spores stored in spent sporulation medium.
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Registered trials
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