ArticleDevelopment genes and evolution2015
Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus.
Article in Development genes and evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Independent evolution toward larger body size in the distinctive Faroe Island mice.G3 (Bethesda, Md.) · 2021Pooled it
- An evolutionarily conserved N-terminal domain of RRF-3 governs GTSF-1 binding in nematodes.EMBO reports · 2026Article
- A multifunctional PKS-NRPS megasynthetase in nematodes produces divergent signaling molecules that control different developmental arrests.Nature communications · 2026Article
- Vitamin B12 induces memory of predation through vitellogenin provisioning.Nature communications · 2026Article
- Article
- Characterization of Heat Shock Protein Expression and Its Application to Temporal Gene Expression in the Nematode Pristionchus pacificus.Development, growth & differentiation · 2026Article
- Description ofJournal of nematology · 2026Article
- Nictation behavior in nematodes.BMC biology · 2025Review
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- Blue light stimulated-autofluorescence green flash of lysosome-related organelles in the intestinal cells of nematodes.G3 (Bethesda, Md.) · 2025Article
- Biosynthesis of modular signaling molecules requires functional diversification of carboxylesterases in Pristionchus pacificus.Communications biology · 2025Article
- Blue light stimulated-autofluorescence green flash of lysosome-related organelles in the intestinal cells of nematodes.bioRxiv : the preprint server for biology · 2025Article
- EBAX-1/ZSWIM8 destabilizes miRNAs, resulting in transgenerational inheritance of a predatory trait.Science advances · 2025Article
- High Nutritional Conditions Influence Feeding Plasticity in Pristionchus pacificus and Render Worms Non-Predatory.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment.Journal of cell science · 2024Article
- Review
- cGMP-dependent pathway and a GPCR kinase are required for photoresponse in the nematode Pristionchus pacificus.PLoS genetics · 2024Article
- Starvation resistance in the nematode Pristionchus pacificus requires a conserved supplementary nuclear receptor.Zoological letters · 2024Article
- Description of two newJournal of nematology · 2024Article
- Co-option of an Astacin Metalloprotease Is Associated with an Evolutionarily Novel Feeding Morphology in a Predatory Nematode.Molecular biology and evolution · 2023Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diplogastrid nematode Pristionchus pacificus is a nematode model system for comparative studies to Caenorhabditis elegans and integrative evolutionary biology aiming for interdisciplinary approaches of evo-devo, population genetics, and ecology. For this, fieldwork can be combined with laboratory studies, and P. pacificus has a well-developed methodological toolkit of forward genetics, whole genome sequencing, DNA-mediated transformation, and various -omics platforms. Here, we establish CRISPR/Cas9-based gene inactivation and describe various boundary conditions of this methodology for P. pacificus. Specifically, we demonstrate that most mutations arise within the first 9 hours after injections. We systematically tested the efficiency of sgRNAs targeting different exons in Ppa-dpy-1 and characterized the molecular nature of the induced mutations. Finally, we provide a protocol that might also be useful for researchers working with other non-Caenorhabditis nematodes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.